Publications by authors named "H Hiroaki"
Article Synopsis
- Macrophages with group V phospholipase A (Pla2g5) produce linoleic acid (LA), which enhances lung inflammation, particularly during viral infections, but their exact function is not fully understood.
- Researchers created specialized mice and stimulated their macrophages to investigate the effects of Pla2g5 and LA on inflammation, using various immune response triggers.
- Findings revealed that Pla2g5 plays a role in lung inflammation by affecting macrophage activity and recruitment, with LA helping to partially restore inflammation when Pla2g5 is absent.
Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), the virus responsible for coronavirus disease 2019 (COVID-19), has caused a global public health crisis. The E protein, a structural protein found in this virus particle, is also known to be a viroporin. As such, it forms oligomeric ion channels or pores in the host cell membrane.
View Article and Find Full Text PDFMolecular mechanisms of amyloid-β peptide fibril and oligomer formation: NMR-based challenges.
Biophys Physicobiol
February 2023
To completely treat and ultimately prevent dementia, it is essential to elucidate its pathogenic mechanisms in detail. There are two major hypotheses for the pathogenesis of Alzheimer's dementia: the β-amyloid (Aβ) hypothesis and the tau hypothesis. The modified amyloid hypothesis, which proposes that toxic oligomers rather than amyloid fibrils are the essential cause, has recently emerged.
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- The study focuses on improving the properties of gelatinized starch by adding agarose, resulting in a stronger gel suitable for immobilizing proteins on microtiter plates.
- It utilizes recombinant proteins with fusion tags, specifically MBP and GST, to detect and quantify protein-protein interactions (PPIs).
- By leveraging the enzymatic activity of GST, the researchers successfully measured dissociation constants of protein interactions using standard microplate equipment, making the method accessible and cost-effective.
The modulation of tight junction (TJ) integrity with small molecules is important for drug delivery. High-dose baicalin (BLI), baicalein (BLE), quercetin (QUE), and hesperetin (HST) have been shown to open TJs in Madin-Darby canine kidney (MDCK) II cells, but the mechanisms for HST and QUE remain unclear. In this study, we compared the effects of HST and QUE on cell proliferation, morphological changes, and TJ integrity.
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