Whole and Purified Aqueous Extracts of L. Seeds Attenuate Apoptosis and the Overproduction of Reactive Oxygen Species Triggered by p53 Over-Expression in the Yeast .

Plants are an important source of pharmacologically active compounds. In the present work, we characterize the impact of black cumin ( L.) aqueous extracts on a yeast model of p53-dependent apoptosis.

Microbiologically influenced corrosion mechanism of 304L stainless steel in treated urban wastewater and protective effect of silane-TiO coating.

Microbiologically influenced corrosion (MIC) of bare and silane-TiO sol-gel coated stainless steel (SS) was studied in treated urban wastewater (TUWW). Combining the electrochemical impedance spectroscopy (EIS) and the scanning vibrating electrode technique (SVET) showed that SS surface colonization occurs, at earlier stages, by iron-oxidizing bacteria (IOB), and later by sulphate-reducing bacteria (SRB). The SVET results showed that chemical corrosion process and bacterial respiration led to the depletion of dissolved oxygen, creating a differential aeration cell and thus a localized corrosion phenomenon.

New Phage Display-Isolated Heptapeptide Recognizing the Regulatory Carboxy-Terminal Domain of Human Tumour Protein p53.

The transcription factor tumor protein p53 (P53) controls a variety of genes most involved in cell cycle and is at the origin of apoptosis when DNA is irreparably damaged. We planned to select novel tumor protein p53-interacting peptides through the screening of hepta-peptide phage-display libraries. For this aim, human tumor suppressor protein p53 was expressed in Escherichia coli as Glutathione S-transferase fusion and purified by affinity chromatography.

Enzymatic hydrolysis of pretreated Alfa fibers (Stipa tenacissima) using β-d-glucosidase and xylanase of Talaromyces thermophilus from solid-state fermentation.

This work aims at realizing an optimal hydrolysis of pretreated Alfa fibers (Stipa tenacissima) through the use of enzymes produced from Talaromyces thermophilus AX4, namely β-d-glucosidase and xylanase, by a solid state fermentation process of an agro-industrial waste (wheat bran supplemented with lactose). The carbon source was firstly selected and the optimal values of three other parameters were determined: substrate loading (10g), moisture content (85%) and production time (10days); which led to an optimized enzymatic juice. The outcome was then supplemented with cellulases of T.

Purification and biochemical characterization of a novel thermostable lichenase from Aspergillus niger US368.

New β-1,3;1,4-glucanase was purified from Aspergillus niger US368. The pure glucanase has a molecular mass of about 32 kDa. The N-terminal sequence of the purified enzyme (A-G-T-N-P-P-I-G-V) was determined.