Background: Increasingly, sheep breeders are using artificial insemination to produce lambs, so finding methods that preserve ram sperm can be useful.
Objective: To determine the protective effects of different concentrations of laminarin on ram sperm motility, viability, abnormalities, membrane, and DNA integrity, superoxide dismutase enzyme (SOD) activity, and malondialdehyde (MDA) production after freeze-thawing.
Materials And Methods: The ejaculates of four rams were collected and stored at 35 degree C.
During the cryopreservation of sperm, the production of highly reactive oxygen species (ROS) can reduce their viability and fertility. However, the addition of antioxidants can help reduce the harmful effects of ROS. One such antioxidant is selenium, which is a co-factor of the glutathione peroxidase enzyme that is effective in scavenging ROS.
View Article and Find Full Text PDFThis study aimed to investigate the association between polymorphisms of and genes and early embryo development of Sanjabi sheep. Blood and ovarian samples were collected from a local slaughterhouse. The cumulus-oocyte complexes with a diameter greater than 3 mm were aspirated from follicles, and maturation (IVM) and culture (IVC) rates of them were recorded.
View Article and Find Full Text PDFBackground: Extensive use of different nanoparticles caused significant concerns about their biological safety.
Objective: This study aimed to evaluate the effects of cryopreservation on ram semen after adding magnetic nanoparticles (MNPs) to separate X and Y chromosome-bearing spermatozoa.
Methods: The experimental ram sperms in this research included treated spermatozoa (50 μg/ml MNPs) and non-treated spermatozoa.