Rapid identification of lettuce seed germination mutants by bulked segregant analysis and whole genome sequencing.

Lettuce (Lactuca sativa) seeds exhibit thermoinhibition, or failure to complete germination when imbibed at warm temperatures. Chemical mutagenesis was employed to develop lettuce lines that exhibit germination thermotolerance. Two independent thermotolerant lettuce seed mutant lines, TG01 and TG10, were generated through ethyl methanesulfonate mutagenesis.

Capturing flavors from Capsicum baccatum by introgression in sweet pepper.

Biochemical characterization in combination with genetic analyses in BC 2 S 1 plants and near-isogenic lines led to the detection and validation of C. baccatum loci affecting flavor, terpenoid content and Brix level. The species Capsicum baccatum includes the most common hot peppers of the Andean cuisine, known for their rich variation in flavors and aromas.

'Quod scripsi, scripsi.' The quality of the report of telephone consultations at Dutch out-of-hours centres.

Objective: To assess the quality of the content of reports of telephone consultations at out-of-hours centres and to investigate to what extent the reports reflect the actual telephone consultation.

Design And Setting: Cross-sectional qualitative study; 17 out-of-hours centres in The Netherlands.

Method: To assess the quality of the content of reports, a focus group developed the Reason for calling, Information gathered, Care advice given, Evaluation of the care advice with the patient (RICE) report rating instrument.

Quality of communication during telephone triage at Dutch out-of-hours centres.

Objective: To assess the quality of communication skills of triagists, working at out-of-hours (OOH) centres, and to determine the correlation between the communication score and the duration of the telephone consultation.

Methods: Telephone incognito standardised patients (TISPs) called 17 OOH centres presenting different clinical cases. The assessment of communication skills was carried out using the RICE-communication rating list.

Quality of clinical aspects of call handling at Dutch out of hours centres: cross sectional national study.

Objective: To assess the quality of telephone triage by following the consecutive phases of its care process and the quality of the clinical questions asked about the patient's clinical condition, of the triage outcome, of the content of the home management advice, and of the safety net advice given at out of hours centres.

Design: Cross sectional national study using telephone incognito standardised patients.

Setting: The Netherlands.