Downregulation of AATK enhances susceptibility to ferroptosis by promoting endosome recycling in gefitinib-resistant lung cancer cells.

Ferroptosis has been characterised by disruption of the cell membrane through iron-related lipid peroxidation. However, regulation of iron homeostasis in lung cancer cells that are resistant to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) remains unclear. Transcriptome analysis identified a significant downregulation of apoptosis-associated tyrosine kinase (AATK) mRNA expression in gefitinib-resistant PC9 (PC9-GR) cells, which were found to be more susceptible to ferroptosis inducers.

Malnutrition and Frailty Are Associated with a Higher Risk of Prolonged Hospitalization and Mortality in Hospitalized Older Adults.

Malnutrition and frailty are independent risk factors of prolonged hospitalization and mortality, respectively. However, the combined association of these conditions with the risk of prolonged hospitalization and mortality in hospitalized elderly patients remains unclear. Our object was to investigate the combined association of malnutrition and frailty on the risk of prolonged hospitalization and mortality in hospitalized elderly patients.

Dominance of Sulfur-Oxidizing Bacteria, , in the Waters Affected by a Shallow-Sea Hydrothermal Plume.

The shallow-sea hydrothermal vent at Guishan Islet, located off the coast of Taiwan, serves as a remarkable natural site for studying microbial ecology in extreme environments. In April 2019, we investigated the composition of prokaryotic picoplankton communities, their gene expression profiles, and the dissolved inorganic carbon uptake efficiency. Our results revealed that the chemolithotrophs spp.

Lower Extremity Stretch-Shortening Cycle Performance in the Vertical and Horizontal Direction as Key Determinants of Success in Collegiate Male Taekwondo.

Background/objectives: The underlying mechanisms of taekwondo-specific jumping ability among different competition levels are still unknown. This study aimed to compare vertical and horizontal stretch-shortening cycle (SSC) performance between athletes of different competitive levels and examine the relationships of force and power production abilities between those two directions in Taiwanese collegiate-level male taekwondo athletes.

Methods: Seventeen male collegiate taekwondo athletes were divided into two groups: medalists (MG, n = 8) and non-medalists (NMG, n = 9); both groups performed countermovement jumps (CMJ) on a force platform and single-leg lateral hops (SLLHs) via an optoelectronic measurement system.

Engineering hydrogen bonding at tyrosine-201 in the orange carotenoid protein using halogenated analogues.

The Orange Carotenoid Protein (OCP) is a unique water-soluble photoactive protein that plays a critical role in regulating the balance between light harvesting and photoprotective responses in cyanobacteria. The challenge in understanding OCP´s photoactivation mechanism stems from the heterogeneity of the initial configurations of its embedded ketocarotenoid, which in the dark-adapted state can form up to two hydrogen bonds to critical amino acids in the protein's C-terminal domain, and the extremely low quantum yield of primary photoproduct formation. While a series of experiments involving point mutations within these contacts helped us to identify these challenges, they did not resolve them.