Intestinal absorption of different types of folate in healthy subjects with an ileostomy.

Our knowledge on the absorption of folate is incomplete. The deconjugation process as a possible limiting factor in the absorption of folates was investigated. The study also attempted to validate the use of the area under the serum response curve (AUC) from food compared with folic acid as a proxy variable for food folate bioavailability.

Folate intake of the Dutch population according to newly established liquid chromatography data for foods.

Background: Determining folate intake is difficult because existing folate data in food-composition tables are scarce and unreliable.

Objective: The purposes of this study were first to analyze 125 of the most important foods that contribute to folate intake in the Netherlands and second to estimate the folate intake of a representative sample of the population.

Design: We analyzed the folate content of foods by using a newly developed HPLC trienzyme method combined with an affinity chromatography cleanup step.

Liquid chromatographic determination of tocopherols and tocotrienols in margarine, infant foods, and vegetables.

A liquid chromatographic (LC) method was developed for determination of tocopherols and tocotrienols in foods. Tocopherols and tocotrienols were released after saponification of test portions for 40 min at 80 degrees C, followed by extraction with hexane-ethyl acetate. After evaporation of the organic solvents, the extract was injected in the LC system.