In vitro study on histamine-induced cyclic adenosine monophosphate production in ulcer patients with different antisecretory responses to ranitidine.

Failure of acid suppression by H2 receptor antagonists has been observed in some patients with peptic ulcer diseases. The reasons for the drug resistance are unknown. In the present study, the effects of histamine and the H2 receptor antagonist ranitidine (CAS66357-35-5) on cyclic adenosine monophosphate (AMP) production was investigated using intact cells from gastric mucosal biopsies derived from patients with adequate (AR) and inadequate (IR) antisecretory response to ranitidine.

A method to assess histamine-induced cyclic AMP production in isolated gastric mucosal cells from human biopsies.

Human gastric mucosal cells were isolated by digestion of fundic biopsies with pronase and collagenase. The mean value of gastric cells per milligram biopsy specimen +/- SEM was 59,000 +/- 4,300 (n = 31) with a viability of 90 +/- 5%. With the cell yield of 1 patient a series of approximately 70-80 cyclic AMP measurements was possible.