Consistent Biofilm Formation by Isolated From Patients With Necrotizing Soft Tissue Infections.

Objectives: Biofilm formation has been demonstrated in muscle and soft tissue samples from patients with necrotizing soft tissue infection (NSTI) caused by , but the clinical importance of this observation is not clear. Although M-protein has been shown to be important for biofilm formation in , the evidence for an association between type and biofilm forming capacity is conflicting. Here we characterize the biofilm forming capacity in a collection of isolates causing NSTI, and relate this to type of the isolates and clinical characteristics of the patients.

Repeated bronchoscopy in health and obstructive lung disease: is the airway microbiome stable?

Objective: Little is known concerning the stability of the lower airway microbiome. We have compared the microbiota identified by repeated bronchoscopy in healthy subjects and patients with ostructive lung diseaseases (OLD).

Methods: 21 healthy controls and 41 patients with OLD completed two bronchoscopies.

Label-free quantitative proteomic analysis of the oral bacteria Fusobacterium nucleatum and Porphyromonas gingivalis to identify protein features relevant in biofilm formation.

Background: The opportunistic pathogens Fusobacterium nucleatum and Porphyromonas gingivalis are Gram-negative bacteria associated with oral biofilm and periodontal disease. This study investigated interactions between F. nucleatum and P.

Immunochemistry-Based Diagnosis of Extrapulmonary Tuberculosis: A Strategy for Large-Scale Production of MPT64-Antibodies for Use in the MPT64 Antigen Detection Test.

Tuberculosis (TB) is a global health problem. The immunohistochemistry (IHC)-based MPT64 antigen detection test has shown promising results for diagnosing extrapulmonary TB in previous studies. However, the anti-MPT64 antibody currently used in the test is in limited supply, and reproduction of a functional antibody is a prerequisite for further large-scale use.