Publications by authors named "H G Schulten"
Mast cell (MCs) activation is the driving force of immune responses in several inflammatory diseases, including asthma and allergies. MCs are immune cells found throughout the body and are equipped with numerous surface receptors that allow them to respond to external signals from parasites and bacteria as well as to intrinsic signals such as cytokines. Upon activation, MCs release various mediators and proteases that contribute to inflammation.
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- - The study investigates how human cord blood-derived mast cells (hCBMCs) respond to the alarmin interleukin-33 (IL-33) by releasing various cytokines, aiming to understand their role in immune responses.
- - Researchers stimulated hCBMCs with different concentrations of IL-33 for varying durations and analyzed the expression and secretion of chemokines and growth factors through microarrays and multiplex assays.
- - Results showed consistent upregulation of certain chemokines and growth factors across all conditions, indicating that mast cells play a crucial role in regulating immune responses and could inform future inflammatory treatment strategies.
Article Synopsis
- - Mast cells are crucial immune cells that react to threats and can release various mediators when activated, particularly in response to the cytokine IL-33, produced during infections or tissue damage.
- - This study focused on how human cord blood-derived mast cells (hCBMCs) respond to different concentrations of IL-33 over short (6 hours) and long (24 hours) periods, discovering changes in cytokine expression.
- - Results showed that acute exposure boosted pro-inflammatory cytokines (like IL-1α, IL-1β) while prolonged exposure led to different cytokines (like IL-5, IL-10) being released, highlighting the unique responses of mast cells depending on the duration of IL-33 stimulation.*
Article Synopsis
- Chemotherapy resistance, particularly to etoposide, significantly contributes to treatment failure in acute myeloid leukemia (AML).
- This study focused on understanding etoposide resistance in AML using HL60 cell lines, identifying three resistant clones (HL60-EtopR H1A, H1B, H1C) with notably higher resistance levels.
- Gene expression profiling revealed the upregulation of src tyrosine kinase family genes in resistant cells, indicating a potential target for further research to overcome resistance using Src inhibitors.
Article Synopsis
- * The research involved isolating CD34 progenitors from umbilical cord blood and differentiating them into MCs over several weeks, assessing their purity through flow cytometry to confirm specific surface markers.
- * Advanced analytical methods, including microarray and gene set enrichment analysis, were utilized to identify unique gene signatures and pathways relevant to the immune response in hCBMCs compared to original CD34 cells.