Systematic design of mouse Vh gene family-specific oligonucleotides.

Kabat's database has often been used to design mouse Vh gene-specific 5' primers. The emphasis was mostly on constructing a universal (degenerate) 5' primer or 5' primer set, which would be able to match every mouse Vh gene. We were interested in finding oligonucleotides that could be used as primers or probes that can discriminate between the different Vh gene families.

RT7-defined alloantigens in rats are part of the leucocyte common antigen family.

Haemopoietic cells carry a variety of cell-surface molecules, some of which are known to have allotypic variation. In rats, the RT7 alloantigenic system has been well documented using alloantisera. We have produced the first mouse hybridoma cell line secreting an antibody, HIS41, which binds to leucocytes of rat strains carrying the RT7.

[Immunomapping in localized bullous pemphigoid].

A 70-year-old Caucasian patient is described, who presented with a bullous dermatosis on an amputation stump. The bullous lesions had developed after practice with a Kondylen-Bettung-Münster (KBM) prosthesis. The lesions disappeared completely after prednisone therapy and replacement of the prosthesis with a Thomas splint.