Evidence of h_{b}(2P)→ϒ(1S)η Decay and Search for h_{b}(1P,2P)→ϒ(1S)π^{0} with the Belle Detector.

We report the first evidence for the h_{b}(2P)→ϒ(1S)η transition with a significance of 3.5 standard deviations. The decay branching fraction is measured to be B[h_{b}(2P)→ϒ(1S)η]=(7.

A novel ADP-directed chaperone function facilitates the ATP-driven motor activity of SARS-CoV helicase.

Helicase is a nucleic acid motor that catalyses the unwinding of double-stranded (ds) RNA and DNA via ATP hydrolysis. Helicases can act either as a nucleic acid motor that unwinds its ds substrates or as a chaperone that alters the stability of its substrates, but the two activities have not yet been reported to act simultaneously. Here, we used single-molecule techniques to unravel the synergistic coordination of helicase and chaperone activities, and found that the severe acute respiratory syndrome coronavirus helicase (nsp13) is capable of two modes of action: (i) binding of nsp13 in tandem with the fork junction of the substrate mechanically unwinds the substrate by an ATP-driven synchronous power stroke; and (ii) free nsp13, which is not bound to the substrate but complexed with ADP in solution, destabilizes the substrate through collisions between transient binding and unbinding events with unprecedented melting capability.

Web Visualization for Spatiotemporal Genomic Epidemiology, Annotation, and Mutation Dynamics of Orthohantavirus hantanense Using Nextstrain.

The phylogeographic inference approach aims to connect genomic data with epidemiology to understand the spread and evolution of pathogens using visualization of spatiotemporal reconstructions. Orthohantavirus hantanense (HTNV), the causative agent of hemorrhagic fever with renal syndrome (HFRS), represents a significant global public health concern. Here, we introduce a localized Nextstrain platform for HTNV, offering a comprehensive resource for facilitating spatiotemporal genomic surveillance and the study of evolutionary dynamics of viral genomes.

Cryopreservation of primary neonatal rat oligodendrocytes and recapitulation of oligodendrocyte characteristics.

Introduction: , primary rat oligodendrocytes (OLs) are widely used for research on OL development, physiology, and pathophysiology in demyelinating diseases such as multiple sclerosis. Primary culture methods for OLs from rats have been developed and improved over time, but there are still multiple aspects in which efficiency can be boosted.

Methods: To make use of excess oligodendrocyte progenitor cells (OPCs) from primary cultures, a cryopreservation process utilizing a commercially available serum-free cryopreservation medium was established to passage and freeze OPCs at -80°C for later use.

High-Density CuBiO Photocathodes Using Well-Textured Buffer Layers and Their Unassisted Solar Hydrogen Production Performances.

Solar hydrogen production using photoelectrochemical (PEC) cells requires the selection of cost-effective materials with high photoactivity and durability. CuBiO photocathodes possess an appropriate bandgap for efficient hydrogen production. However, their performance is limited by poor charge transport and interface voids formed due to the porous structure during annealing, which complicates the deposition of passivation overlayers.