[Effect of redox potential on culture parameters of Trypanosoma cruzi developed in liquid stirred media].

The redox potential (Eh) is a physico-chemical property presented by solutes able to interchange electrons with an inert electrode. The redox potential influences bacterial growth in an independent way from dissolved oxygen. The available information about protozoans in vitro grown is scarce, being Trypanosoma cruzi main example.

On the occurrence of thioredoxin in Trypanosoma cruzi.

The full coding sequence for thioredoxin from Trypanosoma cruzi (TcTRX) strain Tulahuen O has been cloned into the pRSETA vector. The protein was expressed in Escherichia coli with an N-terminal extension of six histidine residues for purification through metal ion chromatography. The biological activity of recombinant TcTRX was proved utilizing the insulin reduction assay.