Publications by authors named "H F Kaspar"
Antimicrobial susceptibility testing (AST) in the veterinary sector by broth microdilution is mainly based on commercially available microtitre plates with specific panels. A critical review of commercially available microtitre panels identified AST panels that fulfil the requirements for obtaining reliable AST results by covering the necessary antimicrobial concentrations for both clinical breakpoints as well as quality control (QC) ranges for approved QC strains. However, there are AST panels in which these prerequisites are only in part fulfilled, and some AST panels that do not fulfil the aforementioned criteria at all.
View Article and Find Full Text PDFParenteral antimicrobial treatment results in the excretion of antimicrobial-resistant bacteria. Dairy cows are commonly housed side by side in tie-stalls and often receive antimicrobial treatment. However, studies investigating treated cows as source of colonization of neighboring cows with resistant bacteria are scarce.
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- The study aimed to analyze the macrolide resistance of porcine Pasteurella multocida isolates in Germany from 2008 to 2021.
- Out of 1114 isolates, only four (0.36%) showed macrolide resistance, with some carrying specific mutations or resistance genes.
- A new element called Tn7730 was discovered in one isolate from 2021, which contained multiple resistance genes, prompting concerns for future monitoring.
Article Synopsis
- Extended-spectrum cephalosporins (ESCs) are crucial antimicrobials used to combat multidrug-resistant (MDR) bacterial infections, but resistance against them is rising, mainly due to certain β-lactamases produced by Enterobacterales.
- This study focused on analyzing Escherichia coli strains with resistance genes collected from Canada, France, and Germany over a 14-year period, employing advanced sequencing techniques to examine the transmission of these resistance genes via plasmids.
- Five distinct plasmid subtypes linked to the spread of ESC resistance genes were identified, with varying prevalence across geographic regions and host species, highlighting the role of plasmid diversity in the global issue of antibiotic resistance.
Data collected from the German national resistance monitoring program GE-Vet showed slowly increasing prevalence of macrolide resistance among bovine respiratory disease (BRD)-associated from cattle over the last decade. The focus of this study was to analyze the genetic basis of antimicrobial resistance (AMR) and the prevalence of multidrug-resistance (MDR)-mediating integrative and conjugative elements (ICEs) in 13 German BRD-associated isolates collected between 2009 and 2020 via whole-genome sequencing. Antimicrobial susceptibility testing (AST) was performed via broth microdilution according to the recommendations of the Clinical and Laboratory Standards Institute for the macrolides erythromycin, tilmicosin, tulathromycin, gamithromycin, tildipirosin, and tylosin as well as 25 other antimicrobial agents.
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