Publications by authors named "H Esumi"
To better understand the disruptions of transcriptional regulations and gene expression in lung cancers, we constructed a multi-omics catalogue of the responses of lung cancer cells to a series of chemical compounds. We generated and analyzed 3,240 RNA-seq and 3,393 ATAC-seq libraries obtained from 23 cell lines treated with 95 well-annotated compounds. To demonstrate the power of the created multi-omics resource, we attempted to identify drugs that could induce the designated changes alone or in combination.
View Article and Find Full Text PDFTo enable the widespread application of genomic medicine, the extraction of genomic DNA from thin sections of archived formalin-fixed and paraffin-embedded (FFPE) tissue blocks for next-generation sequencing (NGS) is often necessary. However, there are currently no guidelines available on which specific regions of the microtome sections to use for macrodissection with respect to the histopathological factors observed under microscopic examination. The aim of this study was to clarify the relationship between histopathological factors and DNA quality, and to standardize the macrodissection method for more efficient implementation of NGS.
View Article and Find Full Text PDFArticle Synopsis
- Humans tend to prefer fatty foods, and if consumed in excess, it can lead to obesity; this study looked into how different amounts of fat affect appetite and biological reactions.
- The trial involved healthy adults with an average age of 29 and body mass index (BMI) of around 21, where participants were given meals with varying butter amounts (0g, 10g, 20g, and 40g) alongside other basic foods.
- Results showed that while consuming butter didn’t significantly impact glucose or insulin levels, those who ate meals with 20-40g of butter reported feeling fuller, particularly after the B40 meal, indicating an increased appetite sensation despite no change in overall satisfaction.
The signaling mechanisms mediating myocardial glucose transport are not fully understood. Sucrose nonfermenting AMP-activated protein kinase (AMPK)-related kinase (SNARK) is an AMPK-related protein kinase that is expressed in the heart and has been implicated in contraction-stimulated glucose transport in mouse skeletal muscle. We first determined if SNARK is phosphorylated on Thr , a site critical for SNARK activity.
View Article and Find Full Text PDFArctigenin is evaluated for antitumor efficacy in patients with pancreatic cancer. It has an inhibitory activity on mitochondrial complex I.Therefore, plasma lactate level of patients after arctigenin administration was evaluated for biomarker of clinical response and/or adverse effect.
View Article and Find Full Text PDF