Gene-editing in patient and humanized-mice primary muscle stem cells rescues dysferlin expression in dysferlin-deficient muscular dystrophy.

Dystrophy-associated fer-1-like protein (dysferlin) conducts plasma membrane repair. Mutations in the DYSF gene cause a panoply of genetic muscular dystrophies. We targeted a frequent loss-of-function, DYSF exon 44, founder frameshift mutation with mRNA-mediated delivery of SpCas9 in combination with a mutation-specific sgRNA to primary muscle stem cells from two homozygous patients.

Evaluation of Pan-RAF Inhibitor LY3009120 on Human Uveal Melanoma Cell Line 92-1.

Background/aim: Uveal melanoma (UM) represents a prevailing primary intraocular malignancy, with a limited median overall survival among metastatic patients, and most tumors lack RAF/RAS mutations. The pan-RAF inhibitor LY3009120 has demonstrated valuable anti-tumor effects in a wide range of RAF/RASmut and wild-type (WT) tumor models. This study aimed to evaluate the antitumor effect of LY3009120 on 92-1 UM cell line.

Dengue Burden and Factors Influencing Severity in Honduras: A Descriptive and Analytical Study.

Background: Dengue is a disease that accounts for a major morbidity and mortality in Honduras.

Methods: This descriptive study used an analytical component based on the data from the National Virology Laboratory between 2016-2022. Ordinal logistic regression was used to identify the factors associated with the classification of dengue without warning signs (DWOS), dengue with warning signs (DWS), and severe dengue (SD).

Effect of In Vitro Micropropagation on the Chemical, Antioxidant, and Biological Characteristics of Sch. Bip., an Endemic Plant of the Atacama Desert Andean Region.

The species Sch. Bip., commonly called "chachacoma", is widely used as a medicinal plant by the Andean communities of Northern Chile.

Mating dynamics of a sperm-limited drosophilid, Zaprionus indianus.

When males have large sperm, they may become sperm limited and mating dynamics may be affected. One such species is Zaprionus indianus, a drosophilid that is an introduced pest species in the Americas. We examined aspects of mating behavior in Z.