A preclinical study of allogeneic CD19 chimeric antigen receptor double-negative T cells as an off-the-shelf immunotherapy drug against B-cell malignancies.

Objectives: To evaluate the manufacturability, efficacy and safety of allogeneic CD19 chimeric antigen receptor double-negative T cells (CD19-CAR-DNTs) as an off-the-shelf therapeutic cell product.

Methods: A membrane proteome array was used to assess the off-target binding of CD19-CAR. DNTs derived from healthy donors were transduced with lentiviral vectors encoding the CD19-CAR.

An analysis of different modalities of bone mineral densitometry evaluation in cage subsidence in anterior cervical discectomy and fusion.

Objective: To compare the effectiveness of different measurement methods on bone miner density (BMD), including cervical HU of CT, MRI-based cervical vertebral bone quality (C-VBQ), and value of DEXA, for predicting cage subsidence after single-level ACDF.

Methods: This is a retrospective study, and patients who underwent single-level ACDF from June 2019 to June 2022 were recruited. We collected preoperative total segmental vertebral height (pre-TSVH), cage subsidence height, cervical angle (CA), T1-slope, straight or reverse cervical curvature, mean HU value of C3-7 (C-HU), mean HU of segment (seg-HU), C-VBQ, segmental C-VBQ (seg-VBQ), and total lumbar value ( value).

Real-world effectiveness and causal mediation study of BNT162b2 on long COVID risks in children and adolescents.

Background: The impact of pre-infection vaccination on the risk of long COVID remains unclear in the pediatric population. We aim to assess the effectiveness of BNT162b2 on long COVID risks with various strains of the SARS-CoV-2 virus in children and adolescents, using comparative effectiveness methods. We further explore if such pre-infection vaccination can mitigate the risk of long COVID beyond its established protective benefits against SARS-CoV-2 infection using causal mediation analysis.

Polyketides and alkaloids from the fungus YB4-17 and -Fumiquinazoline J induce apoptosis, paraptosis in human hepatoma HepG2 cells.

Hepatocellular carcinoma (HCC) is one of the most common malignancies. The currently available clinical drugs for HCC frequently cause serious side effects and the treatment outcomes are unsatisfactory. It is urgent to develop effective drugs with high selectivity and low adverse effects for HCC.

Continuous-Flow and Scalable Synthesis of Pd@Pt Core-Shell Nanocrystals with Enhanced Activity toward Oxygen Reduction.

We report a scalable method based on continuous-flow reactors for conformally coating the surfaces of facet-controlled Pd nanocrystals with uniform, ultrathin shells made of Pt. The key to the success of such an approach is the identification of a proper polyol to generate the Pt atoms at a relatively slow rate to ensure adequate surface diffusion and thus the formation of uniform shells in a layer-by-layer fashion. We first demonstrate the concept using the production of Pd@Pt (n = 2-5) core-shell icosahedral nanocrystals and then have the strategy successfully extended to the syntheses of Pd@Pt cubic and octahedral nanocrystals.