Thinking and practices of new methods for breeding wheat with improved nutrient use efficiency.

In the early 1990s, based on China's basic national conditions, Li Zhensheng put forward the concept of sustainable agricultural development that took the path of resource-conserving and high-yield agriculture. He carried out breeding explorations on the efficient use of mineral nutrients by crops and pioneered a new direction for wheat breeding with the goals of "less input, more output, environmental protection, and sustainable development", mainly focusing on improving the absorption and utilization efficiency of phosphorus and nitrogen. He has led and greatly promoted "the Second Green Revolution" in China's agriculture.

The influence of fluid resuscitation strategy on outcomes from dengue shock syndrome: a review of the management of 691 children in 7 Southeast Asian hospitals.

Introduction: The pathognomonic feature of dengue shock syndrome (DSS) is a transient capillary leak syndrome resulting in profound intravascular volume depletion. WHO management guidelines recommend particular parenteral fluid regimens during the critical leakage phase, including synthetic colloid solutions in certain circumstances. We set out to describe the actual fluid management strategies employed in different settings and to investigate relationships with clinical outcomes.

Development of a highly sensitive, high-throughput and automated CRISPR-based device for the contamination-free pathogen detection.

Rapid, portable, and contamination-resistant nucleic acid detection methods are necessary due to the threat posed by emerging viruses to public health and agricultural output. We establish CARE (CRISPR-associated airtight real-time electronic diagnostic device), a novel platform that combines CRISPR-Cas12a with a hermetically sealed microfluidic chip to overcome the limitations of present technologies, which struggle to balance sensitivity, multiplexing, and field applicability. By combining isothermal amplification and CRISPR detection within a hermetically sealed microfluidic chip, CARE eliminates the risk of nucleic acid aerosol contamination while enabling simultaneous high-throughput analysis of seven pathogens.

siRNA-AGO2 complex inhibits bacterial gene translation: A promising therapeutic strategy for superbug infection.

Silencing resistance genes of pathogenic bacteria by RNA interference (RNAi) is a potential strategy to fight antibiotic-resistant bacterial infections. Currently, RNAi cannot be achieved in bacteria due to the lack of RNA-induced silencing complex machinery and the difficulty of small interfering RNA (siRNA) delivery. Here, we show that exosomal siRNAs can be efficiently delivered into bacterial cells and can silence target genes primarily through translational repression without mRNA degradation.

Predictors of poor functional outcome after endovascular treatment in patients with poor-grade aneurysmal subarachnoid hemorrhage.

Objective: Endovascular treatment (EVT) is considered an effective treatment for patients with poor-grade aneurysmal subarachnoid hemorrhage (aSAH). Although the prognosis of these patients seems to have improved in recent years, it is generally still considered poor. We analyzed our data to determine potential predictors of poor prognosis in these patients.