Ruthenium red staining of the neural lamella of the brain of Galleria mellonella.

The outer surface of the neural lamella, the connective tissue ensheathing the brain, shows the ability to bind ruthenium red in the wax moth larva. Ruthenium red-positive material is sensitive to neuraminidase, hyaluronidase and to some extent to phospholipase C, what suggests that the negative charge on the external surface of the neural lamella depends on the presence of the anionic groups of sialic and hyaluronic acids and phospholipids.

In vitro survival and amethopterin effects on the structure of ovarian tissues of the sawfly, Acantholyda nemoralis Thoms.

Whole ovaries of the sawfly, Acantholyda nemoralis Thoms., Tenthredinidae, Hymenoptera, were cultivated by the organ culture technique of Fell in the medium of Jones and Cunningham modified by doubling the contents of salts, sugars and lactalbumin hydrolysate and supplemented with an addition of folate. In the experimental conditions the sawfly ovary survived for several days showing mitoses in the follicular epithelium.