Spindle morphology changes between meiosis and mitosis driven by CK2 regulation of the Ran pathway.

The transition from meiotic divisions in the oocyte to embryonic mitoses is a critical step in animal development. Despite negligible changes to cell size and shape, following fertilization the small, barrel-shaped meiotic spindle is replaced by a large zygotic spindle that nucleates abundant astral microtubules at spindle poles. To probe underlying mechanisms, we applied a drug screening approach using eggs and found that inhibition of Casein Kinase 2 (CK2) caused a shift from meiotic to mitotic-like spindle morphology with nucleation of robust astral microtubules, an effect reproduced in cytoplasmic extracts prepared from eggs.

Pediatric Patch Testing at Mayo Clinic Between 2016 and 2020.

Allergic contact Dermatitis (ACD) is a common condition within the pediatric population. Patch testing is an important way to identify relevant allergens. To provide an update of the common contact allergens seen in children based on patch testing data at our institution from 2016 to 2020.

Trends in Patch Testing With the Mayo Clinic Standard Series, 2017-2021.

Patch testing to a standard series is used to identify culprit allergens in patients with contact dermatitis. The reaction rates evolve over time based on trends in cutaneous exposures by the general population. The aim of this study was to analyze the patch test results of the Mayo Clinic standard series in patients tested from 2017 to 2021.