Publications by authors named "H D Bolte"
Background: Stroke is one of the leading causes of mortality and disability and can be rarely caused by cardiac myxoma.
Case Summary: Here, we report about a 56-year-old man who suffered from a stroke presented with acute dysarthria and left hemiparesis. Magnetic resonance imaging (MRI) of the brain revealed an acute stroke.
Article Synopsis
- The influenza A virus (IAV) genome is segmented and needs a coordinated packaging process into virions, which relies on interactions between viral RNA packaging signals and the viral nucleoprotein (NP).
- Researchers investigated how mutations in packaging signals and specific amino acid changes in the NP affected this packaging process, focusing on lysine residues that can change charge states in host cells.
- The findings suggest that the charge state of these lysines impacts the efficiency of vRNA packaging, indicating that lysine acetylation plays a significant role in coordinating the incorporation of RNA segments into the viral structure.
The influenza A virus genome consists of eight distinct viral RNAs (vRNAs) that are typically packaged into a single virion as an octameric complex. How this genome complex is assembled and incorporated into the virion is poorly understood, but previous research suggests a coordinative role for packaging signals present in all vRNAs. Here, we show that disruption of two packaging signals in a model H7N7 influenza A virus results in a mixture of virions with unusual vRNA content, including empty virions, virions with one to four vRNAs, and virions with octameric complexes composed of vRNA duplicates.
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- - The influenza A virus (IAV) replication requires the packaging of eight unique genomic RNA segments into a viral particle, thought to be influenced by interactions between these RNA segments.
- - Research has identified many potential interactions between vRNA segments using a method called SPLASH, but the actual importance of these interactions for packaging remains unclear.
- - A study shows that specific mutant viruses, which lack several of these identified interactions, can still package the RNA segments as effectively as the regular virus, suggesting these interactions may not be essential for the packaging process.
The genome of influenza A virus (IAV) consists of eight unique viral RNA segments. This genome organization allows genetic reassortment between co-infecting IAV strains, whereby new IAVs with altered genome segment compositions emerge. While it is known that reassortment events can create pandemic IAVs, it remains impossible to anticipate reassortment outcomes with pandemic prospects.
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