Enhanced pullulanase production through expression system optimization and biofilm-immobilized fermentation strategies.

Pullulanase (PUL) plays a crucial role in breaking down α-1,6-glycosidic bonds in starch, a key process in starch processing and conversion. Based on PulB with high enzymatic activity, the expression of PUL in Bacillus subtilis was enhanced by plasmid screening, double promoter optimization, and signal peptide engineering. Furthermore, we innovatively employed a mussel foot protein to enhance the cell adhesion to carriers and utilized biofilm-based cell immobilization technology to optimize the fermentation process and stimulate biofilm formation.

Exploiting unique NP1 interface: Oriented immobilization via electrostatic and affinity interactions in a tailored PDA/PEI microenvironment enhanced by concanavalin A.

Enzyme immobilization techniques are crucial for enhancing enzyme stability and catalytic efficiency. Traditional methods such as physical adsorption and simple covalent binding often fail to maintain enzyme activity and stability. In this study, an innovative multi-level immobilization strategy was proposed to achieve efficient targeted immobilization of nuclease P1 (NP1) by fine-tuning the surface microenvironment.

Growth Mindset and Job Crafting: A Trait Activation Perspective with Job Autonomy as Moderator.

Job crafting benefits both employees and organizations by enhancing employees' health, well-being, and performance. Therefore, it is crucial to investigate the individual factors that encourage job crafting and the conditions under which they operate. Based on Trait Activation Theory, this study examined the relationship between employees' growth mindset and job crafting, as well as the moderating effect of job autonomy on this relationship.

constructed polymer-layer-modified solid electrolyte enables high-performance all-solid-state batteries.

Side reactions between electrolyte and anode hinder the application of solid-state batteries. Here, a polymer-containing composite solid-state electrolyte (LiPSCl@PCSSE) was obtained through polymerization on LiPSCl. The novel electrolyte was indicated to inhibit side reactions, and the pouch cell showed excellent performance, demonstrating its practical application owing to the employment of LiPSCl@PCSSE.

Identification of Angiogenesis-Related Gene Signatures and Prediction of Potential Therapeutic Targets in Ulcerative Colitis Using Integrated Bioinformatics.

Objective: This study aims to clarify angiogenesis mechanisms in ulcerative colitis and identify potential therapeutic targets.

Methods: The Gene Expression Omnibus (GEO) database was used to obtain expression profiles and clinical data for UC and healthy colon tissues. Angiogenesis-related gene sets were acquired from GeneCards.