Predictive Modeling of Gene Expression and Localization of DNA Binding Site Using Deep Convolutional Neural Networks.

Despite the sequencing revolution, large swaths of the genomes sequenced to date lack any information about the arrangement of transcription factor binding sites on regulatory DNA. Massively Parallel Reporter Assays (MPRAs) have the potential to dramatically accelerate our genomic annotations by making it possible to measure the gene expression levels driven by thousands of mutational variants of a regulatory region. However, the interpretation of such data often assumes that each base pair in a regulatory sequence contributes independently to gene expression.

Delivery of Magnolia bark extract in nanoemulsions formed by high and low energy methods improves the bioavailability of Honokiol and Magnolol.

Honokiol (HK) and Magnolol (MG), isomers found in Magnolia officinalis bark extract (MBE), possess bioactive properties attributed to their biphenolic structure. However, their low polarity results in poor oral absorption, limiting their bioavailability. To enhance their systemic absorption after passing through the digestive tract, efficient carrier systems are essential.

A retrospective description of blood and urine alterations in 386 male cats with urethral obstruction in Botucatu, São Paulo, Brazil.

Background: Urinary retention in obstructed male cats leads to changes in blood and urine compounds, which, combined with the time of obstruction, are linked to the worsening of the animal's clinical status.

Aim: This study aimed to describe the primary laboratory changes in male cats with urethral obstruction (UO).

Methods: Medical records of 386 male cats diagnosed with UO and treated at the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine and Zootechnics (FMVZ), UNESP-Botucatu, between 2010 and 2020 were reviewed.

Deciphering regulatory architectures of bacterial promoters from synthetic expression patterns.

For the vast majority of genes in sequenced genomes, there is limited understanding of how they are regulated. Without such knowledge, it is not possible to perform a quantitative theory-experiment dialogue on how such genes give rise to physiological and evolutionary adaptation. One category of high-throughput experiments used to understand the sequence-phenotype relationship of the transcriptome is massively parallel reporter assays (MPRAs).