Analyzing equilibrium water exchange between myocardial tissue compartments using dynamical two-dimensional correlation experiments combined with manganese-enhanced relaxography.

Water compartments were identified and equilibrium water exchange was studied in excised rat myocardium enriched with intracellular manganese (Mn(2+)). Standard relaxographic measurements were supplemented with diffusion-T(2) and T(1)-T(2) correlation measurements. In nonenriched myocardium, one T(1) component (800 ms) and three T(2) components (32, 120, and 350 ms) were identified.

Dynamic water changes in excised rat myocardium assessed by continuous distribution of T1 and T2.

Ischemic changes in excised rat myocardium were followed by series of T1 or T2 measurements from 1 to 60 min after isolated perfusion cessation, and the influence of manganese enhancement was investigated. An inverse Laplace transformation (ILT) of T1 or T2 data was used to resolve the number, time constants, and fractions of tissue water components in a continuous distribution. For T1 distributions, one single tissue component approximately 900 ms was significantly shortened and dispersed by manganese enhancement (25 and 200 microM MnCl2).

Iodinated radiographic contrast media possess antioxidant properties in vitro.

Purpose: To study potential properties of iodinated radiographic contrast media (IRCM) for intravascular use in in vitro free radical generating reactions.

Material And Methods: Superoxide (*O2-) and hydroxyl (*OH) radicals were generated in xanthine oxidase and Fenton reactions. *O2- was assayed by the nitroblue tetrazolium (NBT) method, whereas *OH was assayed by an aromatic hydroxylation (2-hydroxybenzoic acid) method.

Determination of water compartments in rat myocardium using combined D-T1 and T1-T2 experiments.

We have used combined D-T1 and T1-T2 correlation experiments to explore water compartments in rat heart tissue (myocardium). The results show that two main compartments can be identified, which we assign to extracellular (ec) and intracellular (ic) water. The exchange rate of water across the cell membrane was found to be on the order of 0.