Publications by authors named "H Bouamar"
Background: Respiratory viral infections are a major public health challenge and the most diagnosed medical condition, particularly for individuals living in close proximity, like military personnel. We compared the sensitivity and specificity of the Biomeme Franklin and Truelab RT-PCR thermocyclers to determine which platform is more sensitive and specific at detecting SARS-CoV-2 and influenza A and B viruses.
Methodology: RNA extracted from nasopharyngeal swabs of infected and uninfected individuals was tested on the Biomeme Franklin at Lackland and the Truelab at Wright Patterson Air Force bases.
Aims: Ficolin 3 (FCN3) has the highest complement-activating capacity through the lectin pathway and is synthesized mainly in the liver and lung. Yet, its potential molecular mechanism in hepatocarcinogenesis is not fully understood.
Materials And Methods: The expression of FCN3 in hepatocellular carcinoma (HCC) tumor and non-tumor tissues was analyzed by RT-qPCR, Western blotting and immunofluorescence staining assays.
Background: The incidence and mortality rates of hepatocellular carcinoma among Hispanic individuals in the United States are much higher than in non-Hispanic White people. We conducted multi-omics analyses to elucidate molecular alterations in hepatocellular carcinoma among Hispanic patients.
Methods: Paired tumor and adjacent nontumor samples were collected from 31 Hispanic hepatocellular carcinomas in South Texas for genomic, transcriptomic, proteomic, and metabolomic profiling.
Six Transmembrane Epithelial Antigen of Prostate 2 (STEAP2) belongs to a family of metalloreductases, which indirectly aid in uptake of iron and copper ions. Its role in hepatocellular carcinoma (HCC) remains to be characterized. Here, we report that STEAP2 expression was upregulated in HCC tumors compared with paired adjacent non-tumor tissues by RNA sequencing, RT-qPCR, Western blotting, and immunostaining.
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- - The study investigates the heightened rates of hepatocellular carcinoma (HCC) among Hispanics in the U.S. by analyzing tumor and adjacent non-tumor samples from Hispanic patients in South Texas through comprehensive multi-omics approaches.
- - Key findings include high mutation frequencies related to the Wnt/β-catenin pathway and the identification of HCC subtypes, where one subtype showed better survival rates associated with immune activity and lower liver function-related gene activity.
- - The research aims to provide insights into specific molecular features and potential biomarkers for HCC among Hispanic patients, laying groundwork for improved therapeutic strategies.