Publications by authors named "H Barelli"
Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (DHA-PLs) in physiology. Here, we investigated the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) triggered by RhoA inhibition-associated cell spreading. Lipidomic analyses showed that human umbilical vein endothelial cells (HUVECs) subjected to a DHA diet undergo a 6-fold enrichment in DHA-PLs at the plasma membrane (PM) at the expense of monounsaturated oleic acid-containing PLs (OA-PLs).
View Article and Find Full Text PDFBackground Information: Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available.
View Article and Find Full Text PDFPhospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position whereas the -FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsaturation on membrane flexibility and fission is fragmentary.
View Article and Find Full Text PDFArticle Synopsis
- Small phosphatidylcholine liposomes preferentially bind to the Golgi apparatus in cells, indicating the Golgi's strong affinity for curved membranes.
- The study identifies GMAP-210, a cis golgin, as the key player that mediates this interaction by mimicking lipid-packing sensors and favoring smaller liposomes with specific lipid characteristics.
- Reducing GMAP-210 levels or relocating its sensing features to other organelles like mitochondria decreases the Golgi's ability to capture liposomes, suggesting it acts as a filter for transport vesicles based on size and lipid composition.