Publications by authors named "H BOIRON"

After having presented a method to detect antibody coated bacteria, the authors give reports about the first result they obtained with this technic. Among 41 patients they got a positive immunofluorescence in 18 of them, corresponding with a rate of 80% to the clinical and biological explorations. This research about antibody coated bacteria permits to state whether the kidney tissue is affected or not.

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The laboratory of bacteriology of the CHU of Rouen has isolated in twenty years 297 strains of Listeria monocytogenes which are corresponding to 249 cases, 48 new-borns being infected by the same germ as their mother. The listeriosis frequency is increasing ; the disease is recurrent, with a periodicity of four years, but the number of the cases which are observed every year is larger than that which has been observed four years before. The disease seems to affect mainly the women ; in reality, both sexes are sensitive, but some conditions, especially the pregnancy, are favouring the disease burst.

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Listeria monocytogenes releases its antigens during the exponential phase. Electrosyneresis on agarose allows detection of these antigens in the supernatant of broth culture following a 3 h incubation of the strains. This serotyping method seems to be easy, rapid and reproductible.

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The authors present the interest of electrosyneresis for diagnosis of purulent meningitis. Test material is a study about 120 cerebrospinal fluids. Electrosyneresis sometimes permits to identify the germ when microscopic examination and culture are negative or when microscopy is positive and the culture negative.

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