Antioxidants increase the cryoresistance of GM-CFC.

The influence of antioxidative drugs on the cryopreservation of human bone marrow cells was studied. The viability of bone marrow cells was tested after freezing and thawing from--196 degrees C by the growth of GM-CFC in agar culture. The results suggest the ability of antioxidants to protect stem cells against damage caused by freezing and thawing.

[Experimental studies of the sterilization of transplantation material with peracetic acid].

Experimental investigations for sterilization of prepared transplantation material without aseptic conditions were carried out with peracetic acid of different aqueous and alcoholic dilutions. This chemical germicide is qualified yet for the sterilization of tendinous tissue but not for bone tissue (spongiosa and compact substance). The different cellular structures of the test objects condition this behaviour likely.

[Test of the vitality of hypothermically stored and cryopreserved rat hearts following in vivo coronary perfusion].

Experiments were carried out on cryopreservation of rat hearts. Viability of hearts perfused with cryoprotectant containing solutions as estimated by a short-time connection of rewarmed hearts with the circulation of a recipient animal. ECG, contraction and reflow from the perfused heart were measured.