Structural changes in the cardiac microvasculature of the rat in response to acute high glucose levels: a comparison with diabetes.

Objective: Isolated rat hearts were used to determine whether structural changes in the coronary microcirculation caused by diabetes mellitus occur soon after the onset of hyperglycemia and whether early changes might be reversed by return to normoglycemic conditions.

Methods: Three experimental groups of hearts were used. The perfusate was Krebs-Henseleit medium with the addition of (a) 25 mmol/L glucose for 60 minutes, (b) as (a) followed by 5 mmol/L glucose for 30 minutes, and (c) hearts from diabetic rats perfused for 15 minutes.

Microvascular permeability of the isolated rat heart to various solutes in well-oxygenated and hypoxic conditions.

The aim of this study was to investigate the effect of a moderate degree of hypoxia on coronary vascular permeability to various lipophobic solutes. Using the multiple indicator dilution method the permeability-surface area (PS) products were determined for 125I-albumin, 125I-insulin and 57Co-cyanocobalamin in perfused rat hearts (flow approximately 10 ml.min-1.

The influence of hypoxia on transvascular leakage in the isolated rat heart: quantitative and ultrastructural studies.

1. The multiple indicator dilution method was used to study the transvascular movement of gamma-globulin, bovine serum albumin, insulin and cyanocobalamin in the isolated rat heart. 2.

A method for the simultaneous and continuous monitoring of vascular tone and protein leakage in the synovium.

A technique has been developed to monitor changes in synovial vascular tone and leakage of macromolecules, two of the cardinal features of inflammation. The technique permits not only quantitative sequential measurements of the leakage of 125I-albumin from the circulation into the synovium, but also semiquantitative continuous monitoring of the leakage changes. It allows simultaneous continuous monitoring of changes in synovial vascular tone as well.

A synovial amidase acting on tissue kallikrein-selective substrate in clinical and experimental arthritis.

Increased levels of amidase acting on a tissue-kallikrein selective substrate, Val.Leu.Arg.