Development of Two-Step Temperature Process to Modulate the Physicochemical Properties of -lactoglobulin Nanoparticles.

The development of a new manufacturing process, a two-step temperature treatment, to modulate the physicochemical properties of nanoparticles including the size is critical. This is because its physicochemical properties can be key factors affecting the cellular uptake and the bioavailability of bioactive compounds encapsulated in nanoparticles. The aims of this study were to produce (beta-lactoglobulin) -lg nanoparticles and to understand how two-step temperature treatment could affect the formation and physicochemical properties of -lg nanoparticles.