Regenerative Role of Lrig1+ Cells in Kidney Repair.

Key Points: cells exist long term during kidney homeostasis and become activated upon injury, contributing to regeneration. cells and their progeny emerge during tubulogenesis and contribute to proximal tubule and inner medullary collecting duct development. cells expand and differentiate into a mature nephron lineage in response to AKI to repair the proximal tubule.

A practical handbook on single-cell RNA sequencing data quality control and downstream analysis.

Advancements in single-cell analysis have facilitated high-resolution observation of the transcriptome in individual cells. However, standards for obtaining high-quality cells and data analysis pipelines remain variable. Here, we provide the groundwork for improving the quality of single-cell analysis by delineating guidelines for selecting high-quality cells and considerations throughout the analysis.

Cell Type- and Age-Specific Expression of lncRNAs across Kidney Cell Types.

Key Points: We constructed a single-cell long noncoding RNA atlas of various tissues, including normal and aged kidneys. We identified age- and cell type–specific expression changes of long noncoding RNAs in kidney cells.

Background: Accumulated evidence demonstrates that long noncoding RNAs (lncRNAs) regulate cell differentiation and homeostasis, influencing kidney aging and disease.

Pan-cancer single-cell analysis reveals the heterogeneity and plasticity of cancer-associated fibroblasts in the tumor microenvironment.

Cancer-associated fibroblasts (CAFs) are the predominant components of the tumor microenvironment (TME) and influence cancer hallmarks, but without systematic investigation on their ubiquitous characteristics across different cancer types. Here, we perform pan-cancer analysis on 226 samples across 10 solid cancer types to profile the TME at single-cell resolution, illustrating the commonalities/plasticity of heterogenous CAFs. Activation trajectory of the major CAF types is divided into three states, exhibiting distinct interactions with other cell components, and relating to prognosis of immunotherapy.

Characterizing dedifferentiation of thyroid cancer by integrated analysis.

Understanding of dedifferentiation, an indicator of poo prognosis for patients with thyroid cancer, has been hampered by imprecise and incomplete characterization of its heterogeneity and its attributes. Using single-cell RNA sequencing, we explored the landscape of thyroid cancer at single-cell resolution with 46,205 cells and delineated its dedifferentiation process and suppressive immune microenvironment. The developmental trajectory indicated that anaplastic thyroid cancer (ATC) cells were derived from a small subset of papillary thyroid cancer (PTC) cells.