Draft Genome Sequence of Aspergillus oryzae BP2-1, Isolated from Traditional Malted Rice in South Korea.

The fungus strain BP2-1 was isolated from the traditional malted starter culture nuruk. We report here the draft whole-genome sequence of BP2-1, which is comprised of 14 scaffolds with a total length of 39,455,382 bp and a GC content of 47.13%.

The enhancing effect of Acanthopanax sessiliflorus fruit extract on the antibacterial activity of porcine alveolar 3D4/31 macrophages via NF-κB1 and lipid metabolism regulation.

Objective: The demand for measures to improve disease resistance and productivity of livestock are increasing, as most countries prohibit the addition of antibiotics to feed. This study therefore aimed to uncover functional feed additives to help enhance livestock immunity and disease resistance, using Acanthopanax sessiliflorus fruit extract (ASF).

Methods: ASF was extracted with 70% EtOH, and total polyphenolic and catechin contents were measured by the Folin-Ciocalteu and vanillin assay, respectively.

Diversity analysis of isolated from natural sources by multilocus sequence typing (MLST).

We used multilocus sequence typing (MLST) to analyze the diversity of natural isolates of , the most important microorganism in alcoholic fermentation. Six loci, ADP1, RPN2, GLN4, ACC1, MET4, and NUP116, in genome were selected as MLST markers. To investigate genetic diversity within , 42 isolated from natural sources in Korea as well as six obtained from Genbank and four industrial were examined using MLST.

Extract of downregulates NF-κB-mediated MMP-9 expression, thereby inhibiting invasive activity in a human breast cancer cell line, MDA-MB-231.

is a Korean traditional alcoholic beverage prepared by fermenting rice starch using . It is known for its health benefits, but the mechanisms underlying its inhibitory effects on cancer remain unclear. Here a 70% ethanol extract of (70EMS) was tested for its inhibitory activities against cancer metastasis.

Development of titanium oxide layer containing nanocrystalline zirconia particles with tetragonal structure: Structural and biological characteristics.

This study investigated the microstructural, mechanical and biological properties of oxide layers containing tetragonal zirconia (t-ZrO2) particles on pure titanium produced by plasma electrolytic oxidation (PEO) process. For this purpose, PEO processes were carried out at an AC current density of 200mA/cm(2) for 180s in potassium pyrophosphate (K4P2O7) electrolytes with and without t-ZrO2 powder. Structural investigations using transmission electron microscopy exhibited that the present nanocrystalline oxide layer evidenced the successful incorporation of a myriad of t-ZrO2 particles working as an intermediate medium to reinforce the adhesion strength between the substrate and oxide layer.

Cyanidin-3-O-sambubioside from Acanthopanax sessiliflorus fruit inhibits metastasis by downregulating MMP-9 in breast cancer cells MDA-MB-231.

Anthocyanins play an important role in physiological functions related to human health. This study aimed to investigate the inhibitory effect of cyanidin-3-O-sambubioside isolated from Acanthopanax sessiliflorus fruit on cancer cell metastasis. The inhibitory effect of cyanidin-3-O-sambubioside on angiogenesis was assessed by chorioallantoic membrane and tube formation assays.

Anti-inflammatory lignans from the fruits of Acanthopanax sessiliflorus.

A new lignan, named acanthosessilin A (1), as well as eight known lignan and lignan glycosides 2-9 were isolated from an ethanolic extract of Acanthopanax sessiliflorus fruits. The chemical structures were determined by spectroscopic methods, including HR-EIMS, 1D NMR (1H, 13C, DEPT), 2D NMR (gCOSY, gHSQC, gHMBC, NOESY), and IR spectroscopy. All isolated compounds were tested for the ability to inhibit LPS-induced nitric oxide production in RAW264.

Bioactive 3,4-seco-Triterpenoids from the Fruits of Acanthopanax sessiliflorus.

Eight new 3,4-seco-lupane triterpenes and glycosides, acanthosessiligenins I and II (1, 3) and acanthosessiliosides A-F (2, 4-8), as well as six known 3,4-seco-lupane triterpenes (9-14) were isolated from an ethanolic extract of Acanthopanax sessiliflorus fruits. The chemical structures of 1-8 were determined by spectroscopic data interpretation. All isolated compounds were tested for their cytotoxicity against six human cancer cell lines and their ability to inhibit LPS-induced nitric oxide production in RAW 264.

Sterols isolated from Nuruk (Rhizopus oryzae KSD-815) inhibit the migration of cancer cells.

An activity-guided fractionation method was used to isolate anticancer components from Nuruk (Rhizopus oryzae KSD-815:KSD-815). Dried powder of KSD-815 was extracted with 80% methanol and partitioned successively using nhexane, ethyl acetate, n-butanol, and water. The n-hexane and n-butanol fractions showed a strong antimigratory effect on human cancer cells.

Nuruk extract inhibits lipopolysaccharide-induced production of nitrite and interleukin-6 in RAW 264.7 cells through blocking activation of p38 mitogen-activated protein kinase.

Nuruk, which is a natural inoculator and source of amylolytic enzymes, is used in Korean traditional rice wine. A methanol extract of nuruk (NE) attenuated lipopolysaccharide (LPS)- induced nitrite and interleukin (IL)-6 in RAW 264.7 cells.

Stability of new carbapenem DA-1131 to renal dipeptidase (dehydropeptidase I).

The stability of DA-1131 to renal dipeptidase (RDPase) (EC 3.4.13.