Longitudinal bacterial community dynamics and sodium hypochlorite intervention in a newly built university building.

Urbanisation and building advancements have increased microbial growth in indoor environments, altering human interactions with these microorganisms. Restrooms and their sinks harbour diverse bacterial communities, that differ from those found in natural environments, that could have negative implications for human health. Over two and a half years, this study examined the diversity, temporal dynamics, and resilience of bacterial communities in restroom sink P-traps in a newly built university building.

Dissemination and persistence of antimicrobial resistance (AMR) along the wastewater-river continuum.

Antimicrobial resistance (AMR) is a global health hazard. Although clinical and agricultural environments are well-established contributors to the evolution and dissemination of AMR, research on wastewater treatment works (WwTWs) has highlighted their potential role as disseminators of AMR in freshwater environments. Using metagenomic sequencing and analysis, we investigated the changes in resistomes and associated mobile genetic elements within untreated wastewater influents and treated effluents of five WwTWs, and sediments collected from corresponding river environments in Oxfordshire, UK, across three seasonal periods within a year.

Where is the baby in core knowledge?

What we know about what babies know - as represented by the core knowledge proposal - is perhaps missing a place for the baby itself. By studying the baby as an actor rather than an observer, we can better understand the origins of human intelligence as an interface between perception and action, and how humans think and learn about themselves in a complex world.

Toddlers Prefer Agents Who Help Those Facing Harder Tasks.

Capacities to understand and evaluate others' actions are fundamental to human social life. Infants and toddlers are sensitive to the costs of others' actions, infer others' values from the costs of the actions they take, and prefer those who help others to those who hinder them, but it is largely unknown whether and how cost considerations inform early understanding of third-party prosocial actions. In three experiments ( = 94), we asked whether 16-month-old toddlers value agents who selectively help those who need it most.

Expecting the Unexpected: Infants Use Others' Surprise to Revise Their Own Expectations.

Human infants show systematic responses to events that violate their expectations. Can they also revise these expectations based on others' expressions of surprise? Here we ask whether infants ( = 156, mean = 15.2 months, range: 12.

This too shall pass, but when? Children's and adults' beliefs about the time duration of emotions, desires, and preferences.

This research investigated children's and adults' understanding of the mind by assessing beliefs about the temporal features of mental states. English-speaking North American participants, varying in socioeconomic status (Study 1: N = 50 adults; Study 2: N = 112, 8- to 10-year-olds and adults; and Study 3: N = 116, 5- to 7-year-olds and adults; tested 2017-2022), estimated the duration (seconds to a lifetime) of emotions, desires (wanting), preferences (liking), and control trials (e.g.

Open dataset of theory of mind reasoning in early to middle childhood.

Theory of mind (ToM) reasoning refers to the process by which we reason about the mental states (beliefs, desires, emotions) of others. Here, we describe an open dataset of responses from children who completed a story booklet task for assessing ToM reasoning ( = 321 3-12-year-old children, including 64 (neurotypical) children assessed longitudinally and 68 autistic children). Children completed one of two versions of the story booklet task (Booklet 1 or 2).

A pile of pipelines: An overview of the bioinformatics software for metabarcoding data analyses.

Environmental DNA (eDNA) metabarcoding has gained growing attention as a strategy for monitoring biodiversity in ecology. However, taxa identifications produced through metabarcoding require sophisticated processing of high-throughput sequencing data from taxonomically informative DNA barcodes. Various sets of universal and taxon-specific primers have been developed, extending the usability of metabarcoding across archaea, bacteria and eukaryotes.

Socially intelligent machines that learn from humans and help humans learn.

A hallmark of human intelligence is the ability to understand and influence other minds. Humans engage in inferential social learning (ISL) by using commonsense psychology to learn from others and help others learn. Recent advances in artificial intelligence (AI) are raising new questions about the feasibility of human-machine interactions that support such powerful modes of social learning.

Children seek help based on how others learn.

Do children consider how others learned when seeking help? Across three experiments, German children (N = 536 3-to-8 year olds, 49% female, majority White, tested 2017-2019) preferred to learn from successful active learners selectively by context: They sought help solving a problem from a learner who had independently discovered the solution to a previous problem over those who had learned through instruction or observation, but only when the current problem was novel, yet related, to the learners' problem (Experiment 1). Older, but not younger, children preferred the active learner even when she was offered help (Experiment 2), though only when her discovery was deliberate (Experiment 3). Although a preference to learn from successful active learners emerges early, a genuine appreciation for process beyond outcome increases across childhood.

Microbial diversity and antimicrobial resistance in faecal samples from acute medical patients assessed through metagenomic sequencing.

Antimicrobial resistance (AMR) is a threat to global public health. However, unsatisfactory approaches to directly measuring the AMR burden carried by individuals has hampered efforts to assess interventions aimed at reducing selection for AMR. Metagenomics can provide accurate detection and quantification of AMR genes within an individual person's faecal flora (their gut "resistome").

Young children infer and manage what others think about them.

We care about what others think of us and often try to present ourselves in a good light. What cognitive capacities underlie our ability to think (or even worry) about reputation, and how do these concerns manifest as strategic self-presentational behaviors? Even though the tendency to modify one's behaviors in the presence of others emerges early in life, the degree to which these behaviors reflect a rich understanding of what others think about the self has remained an open question. Bridging prior work on reputation management, communication, and theory of mind development in early childhood, here we investigate young children's ability to infer and revise others' mental representation of the self.

The interactions and hierarchical effects of long-term agricultural stressors on soil bacterial communities.

Soils are subjected to multiple anthropogenic modifications, but the synergistic impacts of simultaneous environmental stressors on below-ground communities are poorly understood. We used a large-scale (1152 plots), long-term (26 years), multi-factorial grassland experiment to assess the impact of five common agricultural practises (pesticides, herbicide, liming, fertilizers and grazing exclusion) and their interactive effects on the composition and activity of soil microbial communities. We confirmed that pH strongly impacts belowground communities, but further demonstrate that pH strongly mediates the impacts of other management factors.

Accumulation of nylon microplastics and polybrominated diphenyl ethers and effects on gut microbial community of Chironomus sancticaroli.

Microplastics (MP) are emerging contaminants with the capacity to bind and transport hydrophobic organic compounds of environmental concern, such as polybrominated diphenyl ethers (PBDEs). The aim of this study was to investigate the ingestion of nylon (polyamide) MP alone and when associated with PBDEs and their effects on Chironomus sancticaroli larvae survival and microbiome structure. Survival, PBDE uptake and microbial community composition were measured in fourth instar larvae exposed for 96 h to BDEs- 47, 99, 100 and 153 in the presence and absence of 1% w/w MP in sediment.

Learning from other minds: An optimistic critique of reinforcement learning models of social learning.

Reinforcement learning models have been productively applied to identify neural correlates of the value of social information. However, by operationalizing social information as a lean, reward-predictive cue, this literature underestimates the richness of human social learning: Humans readily go beyond action-outcome mappings and can draw flexible inferences from a single observation. We argue that computational models of social learning need , i.

The effects of information utility and teachers' knowledge on evaluations of under-informative pedagogy across development.

Teaching is a powerful way to transmit knowledge, but with this power comes a hazard: When teachers fail to select the best set of evidence for the learner, learners can be misled to draw inaccurate inferences. Evaluating others' failures as teachers, however, is a nontrivial problem; people may fail to be informative for different reasons, and not all failures are equally blameworthy. How do learners evaluate the quality of teachers, and what factors influence such evaluations? Here, we present a Bayesian model of teacher evaluation that considers the utility of a teacher's pedagogical sampling given their prior knowledge.

Preoperative Nodal US Features for Predicting Recurrence in N1b Papillary Thyroid Carcinoma.

This study aimed to investigate whether preoperative ultrasonographic (US) features of metastatic lymph nodes (LNs) are associated with tumor recurrence in patients with N1b papillary thyroid carcinoma (PTC). We enrolled 692 patients (mean age, 41.9 years; range, 6-80 years) who underwent total thyroidectomy and lateral compartment LN dissection between January 2009 and December 2015 and were followed-up for 12 months or longer.

A convolutional deep learning model for improving mammographic breast-microcalcification diagnosis.

This study aimed to assess the diagnostic performance of deep convolutional neural networks (DCNNs) in classifying breast microcalcification in screening mammograms. To this end, 1579 mammographic images were collected retrospectively from patients exhibiting suspicious microcalcification in screening mammograms between July 2007 and December 2019. Five pre-trained DCNN models and an ensemble model were used to classify the microcalcifications as either malignant or benign.

Moderated Online Data-Collection for Developmental Research: Methods and Replications.

Online data collection methods are expanding the ease and access of developmental research for researchers and participants alike. While its popularity among developmental scientists has soared during the COVID-19 pandemic, its potential goes beyond just a means for safe, socially distanced data collection. In particular, advances in video conferencing software has enabled researchers to engage in face-to-face interactions with participants from nearly any location at any time.

Beyond knowledge versus belief: The contents of mental-state representations and their underlying computations.

Moving beyond distinguishing knowledge and beliefs, we propose two lines of inquiry for the next generation of theory of mind (ToM) research: (1) characterizing the contents of different mental-state representations and (2) formalizing the computations that generate such contents. Studying how children reason about what others think of the self provides an illuminating window into the richness and flexibility of human social cognition.

Equine grass sickness (a multiple systems neuropathy) is associated with alterations in the gastrointestinal mycobiome.

Background: Equine grass sickness (EGS) is a multiple systems neuropathy of grazing horses of unknown aetiology. An apparently identical disease occurs in cats, dogs, rabbits, hares, sheep, alpacas and llamas. Many of the risk factors for EGS are consistent with it being a pasture mycotoxicosis.

A genomic epidemiological study shows that prevalence of antimicrobial resistance in is associated with the livestock host, as well as antimicrobial usage.

Enterobacterales from livestock are potentially important reservoirs for antimicrobial resistance (AMR) to pass through the food chain to humans, thereby increasing the AMR burden and affecting our ability to tackle infections. In this study 168 isolates from four genera of the order , primarily , were purified from livestock (cattle, pigs and sheep) faeces from 14 farms in the United Kingdom. Their genomes were resolved using long- and short-read sequencing to analyse AMR genes and their genetic context, as well as to explore the relationship between AMR burden and on-farm antimicrobial usage (AMU), in the three months prior to sampling.

Gut and faecal bacterial community of the terrestrial isopod Porcellionides pruinosus: potential use for monitoring exposure scenarios.

This work aimed to characterize the gut and faeces bacterial communities (BC) of Porcellionides pruinosus using high-throughput sequencing. Isopods were collected from the field and kept in laboratory conditions similar to those normally applied in ecotoxicology tests. Faeces and purged guts of isopods (n = 3 × 30) were analysed by pyrosequencing the V3-V4 region of 16 S rRNA encoding gene.

Integration of DNA extraction, metabarcoding and an informatics pipeline to underpin a national citizen science honey monitoring scheme.

Worldwide honeybees ( L.) are one of the most widely kept domesticated animals, supporting domestic and commercial livelihoods through the production of honey and wax, as well as in the delivery of pollination services to crops. Quantifying which plant species are foraged upon by honeybees provides insights into their nutritional status as well as patterns of landscape scale habitat utilization.