[Erwinia amylovora--the fire blight pathogen of trees in Ukraine].

Niduses of fire blight of fruit and ornamental trees have been found in the Kyiv and Vinnitsa regions of Ukraine. Pathogen Erwinia amylovora was isolated between April and October. The pathogen was often accompanied by bacteria Pseudomonas syringae pv.

[Phytopathogenic bacteria of couch-grass in the crops of wheat].

Bacterialdiseases of weeds in the crops of wheat on the fields of Kyiv and Vinnytsya regions of Ukraine Elytrigia repens (L.) Nevski Agropyrum repens L. were revealed.

[Pantoea agglomerans--pathogen of Elytrigia repens and Arrhenatherum elatius diseases].

Strains of Pantoea agglomerans were isolated from the samples with symptoms of bacterial damage of various organs of Elytrigia repens and Arrhenatherum elatius. The isolated bacteria are aggressive to different extent for a host-plant and a number of cultivated plants. Some problems of evolution of this bacterial species parasitism are discussed.

[Pseudomonas genus bacteria on weeds].

It has been shown in the work that the weeds (couch-grass and ryegrass) may be affected by bacterial diseases in natural conditions, Pseudomonas genus bacteria being their agents. The isolated bacteria are highly-aggressive in respect of the host-plant and a wide range of cultivated plants: wheat, rye, oats, barley, apple-tree and pear-tree. In contrast to highly aggressive bacteria isolated from the affected weeds, bacteria-epi phytes isolated from formally healthy plants (common amaranth, orache, flat-leaved spurge, field sow thistle, matricary, common coltsfoot, narrow-leaved vetch) and identified as P.

[Gel-producing properties of exopolysaccharide acrylamide in composition with agar-agar].

A possibility to use a new polymer--exopolysaccharide acrylamide (EPAA)--for partial substitution of agar-agar in solid nutrient media in microbiology has been studied. It has been established than the EPAA in concentration 6.0-12.

[Serological heterogeneity of Pseudomonas syringae pv. atrofaciens strains and their ecological niches].

The paper deals with a comparative analysis of the serological and ecological properties of Pseudomonas syringae pv. atrofaciens strains from the collections of microbial cultures at the Malkov Institute for Plant Genetic Resources and Zabolotny Institute of Microbiology and Virology. All of the strains from the Bulgarian collection, except for one, fall into five serogroups (II through VI) of the classification system of Pastushenko and Simonovich.

[Some characteristics of Pseudomonas syringae pv. maculicola dissociants].

Pseudomonas syringae pv. maculicola dissociants producing colonies of different morphotype were found to possess similar biochemical and serological properties but different virulence to the host plant. The heterogeneous extracellular and intracellular lipopolysaccharide-protein complexes of the dissociants differed in their chemical composition and biological activity towards test plants.

[Effect of bacterial physiological state and heavy metals on the hyperactive reaction of tobacco leaf].

It has been shown that the initiation and proceeding of hypersensitive reaction on the tobacco leaf under the bacterial effect are influenced by the infectious loading of the pathogen, physiological state of bacteria, their viability, temperature of the environment and heavy metal salts. It has been established that the hypersensitive reaction is most distinctly manifested when 1-3 days bacterium cultures are used. Their activity decreases essentially with age, and 10-14 days old cultures are not able to evoke the hypersensitive reaction.

[Epiphytic phase of Erwinia amylovora and Pseudomonas syringae pv. syringae on orchard weeds].

Epiphyte phase of phytopathogenic bacteria Erwinia amylovora and Pseudomonas syringae pv. syringae on the fruit garden weeds has been studied. It has been shown that healthy weeds of the fruit-tree stands can be an ecologic niche for Erwinia amylovora and Pseudomonas syringae pv.

[Adsorbed receptors for Erwinia carotovor subsp. carotovora macromolecular bacteriocins].

Study of nature of receptors for macromolecular bacteriocins Erwinia carotovora subsp. carotovora has shown that lipopolysaccharide (LPS) of cell membrane is an attaching structure for them. It has been established that enzymic treatment of LPS preparation with its further deproteinization by phenol is necessary for isolation of biologically active lipopolysaccharide.

[A rapid method for determining the fatty acid composition of lipid A].

Lipid A is the most conservative part of LPS. Its fatty acids composition can serve as an important taxonomic marker of bacteria. The isolation of LPS and studying their chemical composition are difficult and protracted procedure.

[Characteristics of lipopolysaccharide from Pseudomonas fluorescens (biovar I)].

Lipopolysaccharide (LPS) of the Pseudomonas fluorescens strain IMV 7769 (biovar I) was isolated and investigated. Fractions of the structural parts of the LPS macromolecule, lipid A, the core oligosaccharide, and the O-specific polysaccharide (O-PS), were obtained in a homogeneous state. 2-Hydroxydecanoic, 3-hydroxydecanoic, dodecanoic, 2-hydroxydodecanoic, 3-hydroxydodecanoic, hexadecanoic, octadecanoic, hexadecenoic, and octadecenoic fatty acids were identified in lipid A.

[The chemical composition and biological activity of the glyco polymers in Ralstonia solanacearum (Yabuchi et al., 1995)].

Lipopolysaccharide and extracellular glyco polymers were isolated from cells of phytopathogenic bacteria Ralstonia solanacearum. It was established that the O-specific polysaccharides are characterized by a linear structure, composed of tetrasaccharide repeating units containing three L-rhamnose residues and one of N-acetylglucosamine residue. Core oligosaccharide along with typical monosaccharides such as rhamnose, glucose, heptose, KDO and glucosamine included fucose, galactose and arabinose.

[The characteristics of the lipid A of the lipopolysaccharides in Pseudomonas syringae strains].

Component composition of lipid A of Pseudomonas syringae pv. syringae 218 and P-55, pv. syringae (holci) 8299, pv.

Characterization of lipopolysaccharides from Pseudomonas syringae (serogroup II).

Lipopolysaccharides (LPS) from the strains of Pseudomonas syringae pv.syringae 90a, 435, pv. atrofaciens K-1025, pv.

[The immunochemical characteristics of the lipopolysaccharides of Pseudomonas syringae (pathovars atrofaciens and phaseolicola) and P. holci (serogroup VI)].

Lipopolysaccharides (LPS) of the representatives of strains of serogroup VI Pseudomonas syringae (P. syringae pv. atrofaciens 2399, pv.

[An analysis of the molecular forms of the lipopolysaccharide from Pseudomonas syringae pv. atrofaciens IMV K-1025].

Water extract and salt-EDTA extract of Pseudomonas syringae, pv. atrofaciens cells were fractionated by ultracentrifugation with following salting out of ultracentrifugal supernatant by ammonium sulphate at 55% saturation (pH 4.5).

[Isolation and partial characteristics of biopolymers in the outer membrane of Pseudomonas syringae].

Conditions are developed for obtaining extracellular biopolymers (EBP), water (W), salt-EDTA (SE) and salt-detergent (SD) extracts of Pseudomonas syringae pv. atrofaciens IMV K-1025. The fraction of unsoluble LPS is effectively precipitated from samples by ultracentrifugation (UC).

[The specificity of an immune serum to the exocellular lipopolysaccharide of Pseudomonas wieringae].

The serum obtained to exocellular lipopolysaccharide (ELPS) of Pseudomonas wieringae selectively agglutinated strains of pathovar of P. syringae and did not agglutinated strains of P. cichorii, P.

[Antigenic polysaccharides of bacteria. 37. Structure of the polysaccharide chain of Pseudomonas syringae pv.tabaci (serogroup VII) lipopolysaccharide].

The structure of the O-specific polysaccharide chain of Pseudomonas syringae pv. tabaci strain 223 (serogroup VII) lipopolysaccharide was established on the basis of one- and two-dimensional 1H NMR analysis, 13C NMR analysis and calculation of optical rotation. The structure determined by the non-destructive way was confirmed by acid hydrolysis and methylation.

[The plasmids of Pseudomonas syringae].

The paper deals with occurrence of plasmids in P. syringae strains belonging to seventeen pathovars: the strains were isolated in the USSR and other countries. One to four different plasmids having molecular weights of 20 to 90 Md have been found in various strains of the following twelve pathovars: holci, cerasi, aptata, tabaci, populi, pisi, lupini syringae, lachrymans, phaseolicola, glycinea, atrofaciens.

[Pathogenic characteristics of Pseudomonas aeruginosa].

P. aeruginosa strains isolated from clinical material have been found capable of inducing various types of lesions in agricultural plants: tissue growth, soft and dry rot, changes in the color of plant tissue. In connection with the capacity of P.