MORPHOLOGICAL CHARACTERISTICS OF ANGIOGENESIS IN ADENOCARCINOMA OF COLON.

The study of the process of angiogenesis and microcirculation with the help of modern methods of morphological research including type and maturity of the tumor, location and is considered a problem of special importance and value. The purpose of this research is to study the particularity of angiogenesis and microcirculation in colon adenocarcinomas. The human tissue samples of colon adenocarcinoma (19 cases, 40 observation sites) were studied by light microscopic, electron- microscopic, electron-radiographic and immunohistochemical methods.

VITAMINS D AND E COMBINED EFFECT ON THE RAT MYOCARDIUM UNDER ALLOXAN-INDUCED DIABETES.

It is commonly known that in diabetes mellitus the risk of cardiovascular diseases and mortality increases. The purpose of this study was to determine the specific features of the morphological restructuring of the myocardium in the early stage of experimental diabetes and use the combined impact of powerful antioxidants (Vitamin C and Vitamin E) on the rat myocardium in conditions of alloxan-induced diabetes. The experimental animals were divided into three groups: 40 rats were the target, and 20 rats were the control.

[Morphological characteristic dysplastic features on colonic adenomas].

Morphological changes of tubular adenomas with severe dysplasia are charachterized by low level of cytodifferentiation, epithelial cells intensive proliferation, detected by 3Н-Thymidine and markedly reduction of goblet cells. Functional activity of tumor parenchyma decreases with change in ATP-ase activity. The quantity of young form fibroblasts is markedly increased in adenomous stroma, which caused a disturbance of fibrillogenesis.

[Morphological characteristics of stromal fibroblasts and limphocites in colon and breast cancer].

Stromal substance, lymphocytes (populations and subpopulations) and different mature fibroblasts were studied in the colon and breast cancers by the histology, electron microscopy, and immunocytochemistry and electron radioautographic methods. In cancer stroma marked decrease the total number of T and B cells, in addition to a disturbance of the relations between populations and subpopulations, witch leads to the imbalance of the immune response to tumor growth. In the colon and breast cancer the disturbance of the specific function of fibroblasts and the violation of their cytodifferentiation occur.

Β-cells of rats pancreas and their supposed precursors during alloxan diabetes and after the action of Plaferon LB.

Using the methods of light and electron microscopy, pancreas was studied in the experimental model (Rats-30) of alloxan diabetes - one month from alloxan injection and after the action of Plaferon LB (amniotic interferon). Special emphasis was made on display of extra-islet cells. Data obtained suggest that extra-islet cells exist in the rats pancreas with alloxan diabetes.

[Morphological characterization of experimental parodontitis during its treatment with a phyto-preparation uno de gato].

Examination of new methods of treatment of parodontitis is stipulated by the significant increase in the number of patients with parodontitis. Experiments were conducted on white outbred male rats weighing 150-200 gr, which were divided into three groups. The first group was a reference one (n=20).

[Extra-islet cells of pancreas during the diabetes of alloxan and after the action of plaferon].

Using the methods of light and electron microscopy, pancreas was studied in the experimental model of alloxan diabetes (AD) at various times of the experiment (1 and 6 month) at different degrees of AD and after the action of plaferon. The special emphasis was made on display of compensatory changes that is opportunity of occurrence in extra-islet cells special granules, characteristic for insulin producing B-cells. During the development of pathological processes, there were detected intermediate cells including exocrine and endocrine granules.

[Influence of connective tissue dysplasia on the clinical course of chronic catarrhal gingivitis].

"MASS"- phenotype or dysplasia of the connective tissue has been studied for many years, but it still remains the actual problem in clinical and theoretical medicine. Expansive integration of clinical genetics and molecular biology into the medicine has revealed the most complicated and multistage structure of the pathogenesis of this disease. The aim of our study was focused on the investigation of "MASS" phenotype and its influence on the stomatological disease such as the early stage of periodontitis -- chronic catarrhal gingivitis.

[Morphologic characteristics of microadenomas of the large intestine].

Tissue and cellular atypia, changes of the mucus chemical composition, protein secreting function, ATPase activity, differentiation of the epithelial cells and DNA replication with the widening of proliferation zone were registered morphologically in colon microadenomas. A pronounced increase of labelled small lymphocytes capable of proliferation is observed in the stroma. Fibrocytes are practically absent, fibroblasts are represented by young 3H-labelled forms.

[Hyperplastic changes in the mucosa in colon cancer].

67 segments of rectum and sigmoid removed surgically because of carcinoma were examined: the surface of intestinal mucosa stained with methylene-blue was studied in order to determine the number and localization of focal changes in the macroscopically unchanged areas. Colon microscopy was performed clinically in 24 patients with carcinoma of the rectum and in 24 control individuals. 28 hyperplastic polyps and 1037 microscopic hyperplastic foci were found in operative material.

[The parenchymatous-stromal interrelationships in adenocarcinomas of the large intestine (an electron radioautographic study)].

Parenchymatous and stromal cells in colonic adenocarcinomas were studied electron-radiographically. The properties of the nucleic acid and protein synthesis in the cylindrical and goblet cells in carcinoma (partial 3H-thymidine incorporation, heterogeneity of 3H-uridine and amino acid mixture incorporation) indicate a relatively low level of their differentiation, lower degree of their participation in a specific function, retaining their capacity to proliferate. A high number of 3H-thymidine-labelled stem cells which are the main source of tumour growth is observed in carcinoma.

[Cloacogenic cancer of the rectum].

Three cases of cloacogenous and 35 cases of squamous-cell carcinoma of the distal part of the rectum and anus were studied and compared histochemically to reveal considerable differences in mucopolysaccharides contained by the tumours. Squamous-cell carcinomas were characterized by the presence of glycogen which was not detected in cloacogenous carcinomas. In squamous-cell carcinomas, glycosamine glycanes were represented by sulfo- and sialo-mucin, while cloacogenous carcinomas largely contained a non-sulfated mucosubstance.