Evaluation of biosafe alternative to eosin in hematoxylin and eosin staining procedure: A comparative study.

Background: Hematoxylin and eosin staining is the globally accepted staining technique for histology and histopathology sections. Hematoxylin is a natural dye as compared to eosin which is a synthetic dye manufactured from chemicals. Eosin acts as a counterstain to hematoxylin giving a sharp contrast to its blue color.