Extracerebral infections of Acanthamoeba spp. in mice. pathomorphological changes in tissues of infected animals.

A variety of Acanthamoeba spp. pathogenic strains were isolated from water pools in the area of Poznań. In many cases amoebae invaded lungs, kidney heart, liver and diaphragm causing significant changes in these Organs.

Histochemical study of the nitric oxide synthase activity in experimental trichinellosis.

Nitric oxide plays a critical role in a variety of biological activities. It has been nicknamed a "killer" and "mediator" due to its toxic and signalling properties. Apart from its regular physiological function, nitric oxide indirectly participates in infectious diseases.

New aspects of clinical pathology and electro-physiological muscle disturbances in patients with history of trichinellosis.

Clinical studies performed in 44 patients, one, three, four, six or seven years after they had experienced trichinellosis demonstrated the persistence of various general and motor ailments in 88.7% of the patients. The persisting for many years antibodies against E/S antigen of Trichinella in 86.

Secondary amoebic eye infections in mice due to Acanthamoeba sp.

The aim of the present study was to determine the occurrence of eye infections accompanying the infection of the central nervous system and to demonstrate the possible tissue affinity of different strains of amoebae in subsequent infections. The results obtained demonstrate a clear correlation between the occurrence of eye infection and the degree of virulence of the strains. Amoebae isolated from eyeballs and other organs of dead mice did not exhibit any specificity in relation to the organs in subsequent infections.

Trichinella spiralis: induction of the basophilic transformation of muscle cells by synchronous newborn larvae. II. Electron microscopy study.

The present ultrastructure observations apply to the same experimental groups that were investigated in the first part of the study. The results of electron microscopy investigations correspond to those obtained using light microscopy methods. The ultrastructure data demonstrated that 1-h-old sNBL (group I) penetrated into the sarcoplasm of the muscle cells and transformed it basophilically, finally creating the "nurse cell-muscle larva complex.

Trichinella spiralis: induction of the basophilic transformation of muscle cells by synchronous newborn larvae.

Newborn larvae (NBL) possess the "transformation potential" (TP) to induce the basophilic transformation of the muscle cell into the nurse cell and to create the nurse cell-muscle larva complex. For investigation as to whether TP is age-dependent, three groups of synchronous NBL (sNBL) were obtained at 1 h, 9 h, and 6 days of age, respectively, and were injected intramuscularly into mice. The TP was demonstrated to be high for both 1- and 9-h-old sNBL, but for 6-day-old sNBL, none was found.

Clinical aspects of late sequelae of trichinellosis.

Clinical analysis and the set of laboratory studies, performed in 25 patients one, two, three or six years after surviving acute period of trichinellosis, documented complaints in 22 patients (88.0%) in the form of muscle complaints (68.2%), cardiovascular complaints (45.

Detection of Trichinella SP. invasion and clinical appraisal of patients in the late stage of trichinellosis in a new epidemic focus in Wielkopolska.

A clinical and an epidemiological analysis was presented of a trichinellosis focus from Wielkopolska region. The studies included 20 persons and trichinellosis was diagnosed in 15 cases. The focus was characterized by asynchronous invasion with Trichinella sp.

Histochemical investigations of the biochemical defence mechanism in experimental trichinellosis: II. prostaglandin synthase activity.

In this paper, we report on the histochemical localization of prostaglandin synthase (E. C. 1.

Histochemical investigations of the biochemical defence mechanism in experimental trichinellosis: I. peroxidase activity.

Biological reactions caused by free radicals at the molecular and cellular levels engage many different biochemical components which can be directly damaged by the oxidizing radicals and which eventually promote pathological processes. Defence mechanisms have evolved to limit the speed of production of free radical damage; they include low molecular antioxidants and antioxidant enzymes. In this study, the activity of peroxidase acting against peroxides appearing in muscle of mice at different stages of infection with Trichinella spiralis was analyzed.

Contribution of muscle cell to formation of the capsule around Trichinella spiralis larva. Ultrastructural studies.

In electron microscopic studies on structure of the capsule surrounding Trichinella spiralis larva the techniques have been employed which permit demonstration of proteoglycans and collagen fibers at various stages of their formation, i.e. fixation in tannic acid and staining reaction with ruthenium red.

[The role of the nucleus in the mechanism of transformation of muscle after infection by Trichinella spiralis larvae. III. Morphometric analysis of the functional transformation of the muscle cell nucleus after infection].

Morphometric investigations (using the convergence analysis method) of geometric parameters of nuclei and nucleoli of transformed muscle cells were carried in mice 15 days, 30 days and 6 month after infection with T. spiralis larvae. The analysis showed the largest increase of investigated the parameters of 15th day after the infection.

[The role of the muscle cell nucleus in the mechanism of its transformation after infection by Trichinella spiralis larvae. II. Histochemical features of the functional transformation of the muscle cell nucleus in the course of infection].

The activation of muscle cell nucleus in the course of T. spiralis infection, established using morphological methods (part I), has been confirmed in histochemical (histones, RNP) and histoenzymatic (RN-aze) investigations. The activity of the cell nucleus increased from the 5th day after infection up to the complete encapsulation of the larva (30th day) however it remained at a weak stable level in later stages of infection.

[Morphological assessment of the adaptation process in the small intestine after jejunoileostomy for extreme obesity].

The paper presents the author's experiments performed for examining the morphological adaptation in the intestinal loop, preserved for the food passage after jejunoileostomy for extreme obesity. Macro-, micro-, and ultramicroscopic examinations were carried out in 20 patients. A characteristic elongation and hypertrophy of the active intestinal loop, thickening of its wall (especially mucosa), hypertrophy and elongation of the intestinal villi as well as reduction of the mucous cells could be observed.