Increased complement-mediated leukocytic histamine release in atopics.

Complement-mediated leukocytic histamine release was compared in normal and atopic subjects using isolated leukocytes or heparinized blood. Leukocytes were treated with zymosan-activated autologous serum, whereas blood was directly incubated with zymosan particles. Histamine released from basophils into the leukocyte supernatant or plasma was measured spectrofluorometrically and expressed in percent of total histamine.

Isolation of brush-border membrane from the rabbit descending colon epithelium. Partial characterization of a unique K+-activated ATPase.

The mechanisms of ion movement across the apical membrane of the colon have previously been investigated only in intact tissue. To investigate these mechanisms directly, we have undertaken the isolation and characterization of the apical brush-border membrane of the rabbit descending colon. The purification protocol consists of an initial isolation of single epithelial cells after dissociation of the mucosal layer in EDTA, a high pH (8.

An in vivo demonstration of the antianaphylactic effect of terbutaline.

Allergen-mediated histamine release was measured on small samples of blood in atopic subjects before and following ingestion of two tablets of terbutaline (5 mg) or placebo. No significant variation of histamine release was observed in the placebo group whereas a statistically significant decrease (maximally 45% of basal value) was found in four of the five patients receiving terbutaline. The mean reduction was about 25% of basal allergen-mediated histamine release.