Evaluation of a data-driven youngstock rearing quality system in Dutch dairy herds (2019-2022).

In recent years, the Netherlands exhibited an increased focus on improving youngstock rearing. Several data-driven tools were developed to assess and benchmark calf rearing practices based on key indicators, stimulate farmers to improve their youngstock rearing and reduce calf mortality. KalfOK is such a data-driven tool in which a farm-level overview with 15 indicators of youngstock rearing quality is communicated to participating farmers.

Bovine leukemia virus transmission rates in persistent lymphocytotic infected dairy cows.

Bovine leukemia virus (BLV) establishes a lifelong persistent infection in dairy cattle. White blood cell count (WBC) is correlated with proviral load in the blood and milk of BLV-infected cattle, and testing WBC can be used to assess both BLV infectiousness levels and risk of BLV transmission from different types of infected animals. The objective of the study was to compare effective transmission rates (β) and the basic reproduction ratio ( ) among two types of BLV-infected dairy cows in Chile: those affected with persistent lymphocytosis (PL) vs.

Incidence and recurrence of bovine abortion in dairy cattle from Costa Rica.

The reproductive efficiency and milk yield of cows are crucial factors in a dairy farm's profitability. However, abortions can have a negative impact on these factors. While the morbidity of abortion has been estimated in many countries, information on the burden on dairy cattle in tropical conditions is limited, and Costa Rica is a good example.

Bridging Food Systems and One Health: A key to preventing future pandemics?

Food Systems and One Health are two approaches increasingly known for the holistic perspective they bring when addressing the issues that concern them: food and health. This systematic literature review aims to explore the evidence for using these approaches in a concerted manner to better manage zoonoses. By zoonoses management, we refer to improving the ability to address current zoonoses as well as preventing future ones.

Seroprevalence of pathogenic serogroups in asymptomatic domestic dogs and cats: systematic review and meta-analysis.

Leptospirosis is a neglected zoonotic disease transmitted by contact with the urine of animals infected with pathogenic species of the bacteria or by contact with environments contaminated with the bacteria. Domestic dogs and cats may act as reservoirs or as sentinels of environmental contamination with leptospires, posing a public health concern. There is a great diversity of leptospires, and one common way to classify them is into serogroups that provide some information on the host species they are associated with.

Draft genomes of 16 strains from cats in Valdivia, Chile.

is a primary zoonotic agent, having cats as asymptomatic reservoirs. In humans, it causes cat scratch disease. Here, we report the whole genome sequences of 16 strains isolated from cats in Valdivia city, Southern Chile.

Urine shedding patterns of pathogenic Leptospira spp. in dairy cows.

Pathogenic Leptospira spp. are zoonotic bacteria that infect wild and domestic animals. Humans contract leptospirosis directly through contact with infected animals or indirectly from contaminated water or soil.

spp. in households with cats: Risk factors for infection in cats and human exposure.

The aim of this study was to estimate the occurrence of spp. per household in cats and the risk factors for spp. positivity in cats and their owners from Valdivia, Chile.

Diagnostic accuracy of an immunomagnetic separation-PCR assay to detect pathogenic Leptospira spp. in urine from dairy cattle, using a Bayesian latent class model.

Leptospirosis is a zoonotic disease that has spread worldwide and causes significant economic losses in the dairy industry. The causal agents of this infectious disease are members of the genus Leptospira, known as pathogenic Leptospira spp. Specific clinical signs of the infection are difficult to detect.

Green AOT reverse micelles as nanoreactors for alkaline phosphatase. The hydrogen bond "dances" between water and the enzyme, the reaction product, and the reverse micelles interface.

In this work, we present an investigation of the influence of water encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/methyl laurate and 1,4-bis-2-ethylhexylsulfosuccinate/isopropyl myristate reverse micelles on the enzymatic hydrolysis of 1-naphthyl phosphate by alkaline phosphatase. Our results show that the enzyme is active in the biocompatible reverse micelles studied and that the Michaelis-Menten kinetic model is valid in all systems. We found that both micellar systems studied have a particular behavior toward pH and that the penetration of external solvents into the interfaces is crucial to understanding the effect.

A safe and effective vaccine against bovine leukemia virus.

Previous attempts to develop a vaccine against bovine leukemia virus (BLV) have not been successful because of inadequate or short-lived stimulation of all immunity components. In this study, we designed an approach based on an attenuated BLV provirus by deleting genes dispensable for infectivity but required for efficient replication. The ability of the vaccine to protect from natural BLV infection was investigated in the context of dairy productive conditions in an endemic region.

Speciation and Proton Conductivity of Phosphoric Acid Confined in Mesoporous Silica.

Phosphoric acid (PA) confined in a commercial mesoporous silica (CARIACT G) with porous size in the range of 3 to 10 nm was studied in relation to its coordination with the silanol groups on the silica surface as a function of temperature, up to 180 °C, using P and Si MAS NMR spectroscopy. As the temperature increases, the coordination of Si and P in the mesopores depends on the pore size, that is, on the area/volume ratio of the silica matrix. In the mesoporous silica with the higher pore size (10 nm), a considerable fraction of PA is nonbonded to the silanol groups on the surface, and it seems to be responsible for its higher conductivity at temperatures above 120 °C as compared to the samples with a smaller pore size.

Seroprevalence of pathogenic Leptospira spp. in domestic dogs from southern Chile and risk factors associated with different environments.

Leptospirosis is a neglected and severe zoonotic bacterial disease that affects canine populations worldwide, and it is present in animals from different settings, such as urban and rural environments. Therefore, a cross-sectional study was conducted on owned domestic dogs from urban and rural origins in southern Chile. The study aimed: 1) to estimate the true prevalence of pathogenic Leptospira in dogs from urban and rural environments in southern Chile, 2) to determine the serovars circulating in an endemic area of Chile, 3) to assess potential risk factors associated with seropositivity in dogs from urban and rural environments.

Assessment of Natural Transmission of Bovine Leukemia Virus in Dairies from Southern Chile.

Bovine leukemia virus (BLV) is a retrovirus that affects cattle worldwide. A longitudinal study was conducted with the aim to (a) estimate the incidence rate of the BLV infection of dairy farms in the regions of Los Ríos and Los Lagos (Chile), and (b) describe the frequency and epidemiological association of risk management practices related to new cases in cattle on dairy farms in Southern Chile. Infection status was based on commercial blocking ELISA results, on serum and milk.

Network analysis of cattle movements in Chile: Implications for pathogen spread and control.

Livestock movement between herds is one of the main routes for a pathogen to spread between herds. Understanding the contact structure, patterns and importance of specific individuals and classes can help assess dissemination risk, control, and surveillance measures. In this study, the cattle herd's contact structure in southern Chile was characterized based on the study of movement within one of the most important geographical regions for livestock production.

Assessment of the Current Surveillance System for Human Leptospirosis in Ecuador by Decision Analytic Modeling.

Leptospirosis is a globally disseminated zoonotic disease with no national surveillance systems. On the other hand, surveillance is crucial for improving population health, and surveillance systems produce data that motivates action. Unfortunately, like many other countries, Ecuador put in place a monitoring system that has never been tested.

Comparison of experimental times in T-D and D-T correlation experiments in single-sided NMR.

Diffusion-relaxation correlation experiments in nuclear magnetic resonance are a powerful technique for the characterization of fluid dynamics in confined geometries or soft matter, in which relaxation may be either spin-spin (T) or spin-lattice (T). The general approach is to acquire a set of bidimensional data in which diffusion is codified by the evolution of the magnetization with either Hahn or stimulated echoes (STE) in the presence of a constant magnetic field gradient. While T is codified by a Carr-Purcell-Meiboom-Gil (CPMG) sequence, T is either encoded by saturation or inversion-recovery methods.

Pathogenic spp. Seroprevalence and Herd-Level Risk Factors Associated with Chilean Dairy Cattle.

Leptospirosis is a ubiquitous distributed zoonotic infectious disease present in wild and domestic animals. This study aimed to estimate within-herd and herd-level seroprevalence against pathogenic spp. in dairy cows from southern Chile and identify risk factors associated with the herd-level status.

Bovine Viral Diarrhea Virus within and herd prevalence on pasture-based dairy systems, in southern Chile dairy farms.

Bovine Viral Diarrhea (BVD) is a ubiquitous endemic disease in most bovine populations that causes a negative economic impact on cattle production systems. However, true herd and individual prevalence in Chile have not been estimated based on statistical and epidemiological considerations and uncertainty regarding the infection's extension at animal and herd levels. In addition, the risk factors that can influence how the infection has spread among the cattle premises have not been assessed yet.

Creating High-Resolution Microscopic Cross-Section Images of Hardwood Species Using Generative Adversarial Networks.

Microscopic wood identification plays a critical role in many economically important areas in wood science. Historically, producing and curating relevant and representative microscopic cross-section images of wood species is limited to highly experienced and trained anatomists. This manuscript demonstrates the feasibility of generating synthetic microscopic cross-sections of hardwood species.

Measuring Calves' Usage of Multiple Environmental Enrichment Objects Provided Simultaneously.

This study aims to assess calf usage of five potential enrichment devices provided simultaneously. We used 25 weaned Holstein-Friesian calves housed in groups of five (five replicates), and their behavior was recorded continuously with video cameras. This longitudinal observational study used a pen equipped with a mechanical and fixed brush, cowhide, and horizontal and vertical ropes.

Accurate location of hydrogen atoms in hydrogen bonds of tizoxanide from the combination of experimental and theoretical models.

To obtain detailed information about the position of hydrogen atoms in hydrogen bonds, HBs, of crystalline organic molecular compounds is not an easy task. In this work we propose a combination of ssNMR experimental data with theoretical procedures to get such information. Furthermore, the combination of experimental and theoretical models provides us with well-defined grounds to analyse the strength of π-stacking interactions between layers of hydrogen bonded molecules.