Impact of quercetin, carnosine, and ozone in the cryopreservation on Nellore () semen.

The objective of this study was to reduce the effects of cryoinjury caused in bovine semen by cryopreservation. Ejaculates were collected from Nellore bulls and subjected to freezing in C (control), ozone (15, 30, and 60 µg mL of ozone), quercetin (25, 50, and 100 µg mL of quercetin), and carnosine groups (100, 200, and 300 ng mL of carnosine). Samples were evaluated post-thaw (M0) and post-rapid thermoresistance test (M30) for sperm kinetics (total motility, progressive motility, curvilinear speed, linearity and amplitude of lateral head displacement) and cell structure viability (plasma membrane integrity, acrosomal integrity, mitochondrial potential, membrane fluidity, and lipid peroxidation).

Estradiol Priming Potentiates the Kisspeptin-Induced Release of LH in Ovariectomized Cows.

The present study examined whether priming with estradiol benzoate (EB) for 12 h increased both the peak and duration of LH release in response to kisspeptin (KISS1, KP) in cows. In a Latin square design, ovariectomized Nelore cows ( = 8) received: Control, i.m.

P4/E2-based protocol for synchronisation of ovulation of mares during the breeding and non-breeding season.

Dispersed ovulation at the breeding (BS) and anestrus at non-breeding season (NBS) are major impediments to embryo transfer and insemination programmes. The present study aimed to evaluate a hormonal P4/E2-based synchronisation protocol in mares during both the BS and the NBS on ovarian/follicle behaviour. Mares underwent a hormone protocol to synchronise their ovulation during the BS (n = 8) and NBS (n = 10), starting (D0) with the insertion of an intravaginal device containing 1 g of P4 and 7 mg Estradiol Benzoate IM.

Shading effect on physiological parameters and in vitro embryo production of tropical adapted Nellore heifers in integrated crop-livestock-forest systems.

The aim of this study was to evaluate the impact of increased shadow supply in integrated crop-livestock-forest systems on in vitro embryonic development and physiological parameters related to stress response in Nellore heifers (Bos indicus). For the study, animals (n = 16) were randomly divided into two groups and kept in areas with different afforestation systems, the integrated crop-livestock-forest (ICLF) and the integrated crop-livestock (ICL) system. The microclimate of the ICLF system provided better comfort conditions than ICL.

Administration of PGF during the periovulatory period increased fertilization rate in superovulated buffaloes.

The aim of the present study was to determine the recovery of embryonic structures (ova/embryos) and fertilization rate in superovulated buffaloes treated with PGF during the periovulatory period. On day 0 (D0), buffaloes at random stages of the estrous cycle were treated with an intravaginal progesterone device (P4; 1.0 g) and estradiol benzoate (EB, 2.

Genome-wide association study for age at puberty in young Nelore bulls.

Selection for bulls that would reach puberty early reduces the generation interval and increases fertility and herd productivity. Despite its economic importance, there are few QTL associated with age at puberty described in the literature. In this study, a weighted single-step genome-wide association study was performed to detect genomic regions and putative candidate genes related to age at puberty in young Nelore bulls.

Vitrification of germinal-vesicle stage equine oocytes: Effect of cryoprotectant exposure time on in-vitro embryo production.

Previous studies have found low rates of blastocyst development (0-11%) after vitrification of germinal vesicle (GV)-stage equine oocytes. In this study, we systematically evaluated a short (non-equilibrating) system for GV-stage oocyte vitrification. In Exp.

Human-animal interaction, stress, and embryo production in Bos indicus embryo donors under tropical conditions.

This study investigated the effect of human-animal interaction (HAI) and the stress response on the quality of embryo production in superovulated Nelore (Bos indicus) cattle, under tropical conditions. Thirty-two females underwent a superovulation protocol for 5 days. Cortisol concentrations were determined in blood plasma collected on days 0, 4, and 5.

Evaluation of two regional anesthetic methods on the front limb of dogs using hyperbaric bupivacaine.

Purpose: To evaluate the effects of bupivacaine 0.5 and 0.25% in intravenous regional anesthesia (IVRA) and brachial plexus block (BPB), respectively, on anesthesia, motor block and cardiovascular parameters in dogs.