Publications by authors named "Gustavo A Blanco Heras"
Article Synopsis
- A new technique using indirect photometric detection (IPD) in capillary electrophoresis (CE) was developed, employing a blue LED and the dye Orange G to analyze both inorganic and organic anions sensitively.
- The approach involved using nonaqueous solvents like methanol and DMSO to minimize dye adsorption on the capillary wall, resulting in improved baseline stability and detection performance compared to aqueous systems.
- The optimized method provided significantly lower limits of detection (0.10-0.18 microM) and showcased a sensitivity improvement of two- to six-fold over previous methods, marking it as the most sensitive IPD method for anion detection in CE to date.
This work presents the determination of low-molecular-mass (LMM) organic acids by CE after extraction with MeOH and preconcentration by evaporation. The interference arising from the simultaneous concentration of fast mobile anions (chloride, nitrate, and sulfate) at levels 200 times higher than those of LMM organic acids was reduced by the application of reversed pre-electrophoresis (RPE) as clean-up technique. This methodology allows for an effective elimination of high levels of fast mobile anions from the capillary before the separation has taken place, although analytes are also partially eliminated according to their electrophoretic velocities.
View Article and Find Full Text PDFCapillary electrophoretic method for the determination of inorganic and organic anions in real samples. Strategies for improving repeatability and reproducibility.
J Chromatogr A
March 2007
Selectivity and robustness of the pyromellitic acid (PMA) based background electrolyte was improved in order to increase its applicability for routine analysis of inorganic and organic anions in real samples. An electrolyte composed of 6.75 mM PMA, 0.
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