Perinatal and postnatal patterns of plasma sex steroid-binding protein and testosterone in relation to puberty in the male little brown bat.

The little brown bat (Myotis lucifugus lucifugus) possesses a specific plasma sex steroid-binding protein (SBP) which exhibits high affinity for both testosterone (T) and estradiol. To examine the peri- and postnatal patterns of plasma androgen binding in relation to puberty, concentrations of SBP and total T levels were measured in males at various intervals throughout the first year of life. In addition, reproductive organs were examined histologically over this period.

The influence of treatment with estrogens and estramustine phosphate on platelet aggregation and plasma lipoproteins in non-disseminated prostatic carcinoma.

The treatment of prostatic carcinoma with estrogens is associated with an increased risk of cardiovascular as well as thromboembolic complications. In the present study, patients harboring highly or moderately differentiated prostatic carcinoma without signs of metastases were treated with either polyestradiolphosphate + etinylestradiol, estramustine phosphate or given no treatment. Subsequently, these patients were investigated regarding factors (platelet aggregation, plasma and platelet phospholipid composition and lipoprotein patterns) that might contribute to increased thrombogenesis and cardiovascular risk.

Seasonal variations in pituitary thyrotropes of the hibernating bat Myotis lucifugus lucifugus: an immunohistochemical study.

Pituitary thyrotropes were identified throughout the year in the hibernating bat Myotis lucifugus lucifugus by means of light microscopic immunohistochemistry. These cells occupied a small proportion of the volume of the pars distalis (mean = 1.36% in males; mean = 1.

Seasonal variations in pituitary LH-gonadotropes of the hibernating bat Myotis lucifugus lucifugus: an immunohistochemical study.

Pituitary gonadotropes were identified throughout the year in the seasonally breeding, hibernating bat Myotis lucifugus lucifugus by means of light microscopic immunohistochemistry. In both male and female bats, these cells were immunoreactive with an antiserum directed to the beta subunit of luteinizing hormone. Some gonadotropes were aggregated near a portion of the infundibular stalk which crosses the anterior lobe, while most were scattered singly in a uniform manner throughout the rest of the pars distalis.

Luteinizing hormone-releasing hormone (LH-RH) cells and their projections in the forebrain of the bat Myotis lucifugus lucifugus.

Luteinizing hormone-releasing hormone (LH-RH) neurons and their projections were studied by immunocytochemistry in the brains of little brown bats (Myotis lucifugus lucifugus: Chiroptera: Vespertilionidae ) as a first step in the study of relationships between these neurons and the seasonal reproductive events characteristic of this species. The majority of immunoreactive neurons in adult male, adult female, and fetal bats were ovoid bipolar cells with one thin and one thicker process, both of which gave rise to fine varicose fibers. LH-RH-immunoreactive perikarya were concentrated in the region of the arcuate nuclei in all bats examined.

A quantitative study of pituitary colloid in the bat Myotis lucifugus lucifugus in relation to age, sex, and season.

Extracellular colloid accumulations were examined in pituitary glands of adult and juvenile, male and female, little brown bats, Myotis lucifugus lucifugus (Chiroptera: Vespertilionidae), collected at various times during the annual reproductive cycle. Round or ovoid periodic acid-Schiff-reactive accumulations of colloid were scattered throughout the pars distalis, pars intermedia, and pars tuberalis. These accumulations were surrounded by processes of agranular "follicular" cells.

Effect of protein-reduced diet on plasma lipids, apolipoproteins and lipolytic activities in patients with chronic renal failure.

The effect of treatment with protein-reduced diet on plasma lipids, apolipoproteins and lipolytic activities was studied in 15 patients with chronic renal failure. Mean treatment time was 7.4 months.

Plasma lipoproteins and lipolytic enzyme activities during endurance training in sedentary men: changes in high-density lipoprotein subfractions and composition.

Eighteen healthy sedentary males took part in supervised bicycle training for 50 minutes three to five times a week. Twelve subjects (group A) trained for 6 weeks at heavy intensity, and six subjects (group B) trained for 12 weeks at moderate intensity. Maximal oxygen uptake increased by about 20% (P less than 0.

Plasma adrenocorticotropin and cortisol responses to submaximal and exhaustive exercise.

Adrenocorticotropic hormone (ACTH) levels were compared before and after submaximal and exhaustive isotonic exercise in six normally active college students (3 men and 3 women). Each subject participated in three experiments conducted at the same morning hour. Venous plasma was obtained before and immediately after 20-min runs at 65 and 80% of maximal O2 consumption (VO2 max) and after a run of gradually increasing work intensity which resulted in exhaustion (100% VO2 max) in 12.

In-hospital exercise therapy in patients with severe angina pectoris.

The authors evaluated physiologic, psychologic and metabolic effects of a nine-week in-hospital training program on 14 men with severe disabling angina pectoris. The exercise program consisted of intensive interval training on an ergometer bicycle for two 30 min sessions daily. The physical performance increased by about 40% (p less than 0.

Effects of acetylsalicylic acid and dietary intervention on primary hemostasis.

There is evidence that pathological aggregation of platelets in atherosclerotic arteries is initiated by hemorrhage through fissures in atheromatous plaques. Bleeding time determination reflects in vivo the physiologic function of platelets in their aggregation in injured vessels and can be used as a relevant model for primary hemostasis in investigations with antithrombotic aims. Acetylsalicylic acid is known to cause prolongation of bleeding time by inhibiting prostaglandin biosynthesis.

Control of plasma sex steroid-binding protein in the bat Myotis lucifugus lucifugus: induction of steroid-binding activity in immature males.

Concentrations of sex steroid-binding protein (SBP) in plasma of immature male little brown bats (Myotis lucifugus lucifugus) were found to be low (15 +/- 2.8 (S.E.

Taurocholate is more potent than cholate in suppression of bile salt synthesis in the rat.

Synthesis of bile salts is regulated through negative feedback inhibition by bile salts returning to the liver. Individual bile salts have not been distinguished with regard to inhibitory potential. We assessed inhibition of bile salt synthesis by either cholate or its taurine conjugate in bile fistula rats.

Effects of acetylsalicylic acid on platelet aggregation before and during increase in dietary eicosapentaenoic acid.

The effect of acetylsalicylic acid (ASA) on platelet aggregation before and during a fish diet, already known to decrease the aggregability of platelets and to prolong the bleeding time, was studied in 10 healthy men. Two doses (3.5 and 10 mg/kg body weight) of ASA were given.

Effects of thyrotrophin stimulation for two hours on mouse thyroid cyclic AMP levels in vivo and in vitro.

A thyrotrophin (TSH) stimulation in vivo in mice for 2 h, reflected by continuously increasing plasma triiodothyronine (T3) levels, was associated with an increase in the thyroid content of cyclic AMP (cAMP) during the first 25 min of stimulation; thereafter the level rapidly declined. A similar pattern of the cAMP response was found when mouse thyroid tissue was stimulated by TSH in vitro for 2 h. This is an in vivo demonstration of a type of cAMP response to prolonged hormonal stimulation that has been observed in several in vitro systems including thyroid tissue, generally referred to as hormone induced desensitization of adenyl cyclase.

Influence of sex and obesity on plasma catecholamine response to isometric exercise.

The effects of obesity and gender on plasma catecholamine levels were studied in 10 lean men, 10 lean women, and 10 obese women. Measurements (mean +/- SE) were obtained sequentially after 30 min of bedrest, 10 min of standing, and 5 min of sustained handgrip, and following 15 min of rest while standing. The supine plasma norepinephrine (NE) levels were comparable among the 3 groups, whereas plasma epinephrine (E) was lowest in obese women (14 +/- 2 pg/ml), intermediate in lean women (19 +/- 2 pg/ml), and highest in lean men (29 +/- 5 pg/ml).

Alterations in plasma proteins and lipoproteins in acute myocardial infarction: effects on activation of lipoprotein lipase.

Plasma lipoprotein concentrations were followed in 21 men with acute myocardial infarction. HDL and LDL cholesterol concentrations showed similar time-courses with average maximal decreases of about 20%, 10-14 days after onset of symptoms. The decrease in HDL levels (measured as HDL cholesterol and apolipoprotein AI) was significantly correlated to the inflammatory response, as reflected by plasma orosomucoid concentrations, and to the extent of myocardial injury, as mirrored by serum activities of lactate dehydrogenase.

Metabolic studies in women with previous gestational diabetes during contraceptive treatment: effects on serum lipids and high density lipoproteins.

Triglycerides, cholesterol and phospholipids in serum and high density lipoproteins (HDL) were assessed in 11 women with previous gestational diabetes before and repeatedly during 6 months of low dose progestogen (lynoestrenol = LYN) contraceptive administration. Eight of these women also were followed in an identical manner during non-hormonal contraception (IUD) and 6 of them during combined oral contraceptive administration (EE + LYN). During the use of IUD or LYN administration neither serum nor HDL lipids changed.

Lipid metabolic studies in oophorectomised women: effects on serum lipids and lipoproteins of three synthetic progestogens.

Norethisterone acetate, medroxyprogesterone acetate and levonorgestrel were administered to oophorectomised women to evaluate the effects they have on lipid metabolism. Blood samples were drawn after a 3 wk period without hormone therapy and after 3 wk on each progestogen. Serum and lipoprotein lipids were followed and an oral glucose tolerance test with blood glucose and plasma insulin determinations were performed.

Staining of interphase nuclei and mitotic figures in cultured cells with alcian blue 8GX.

Cultured endothelial cells derived from bovine calf pulmonary artery were subjected to a variety of fixatives and stained with 1% Alcian blue 8GX at pH 2.59 to 3.26.

Factors influencing the release of cyclic AMP from mouse thyroid tissue stimulated by TSH in vitro.

The accumulation of cyclic AMP (cAMP) in mouse thyroid tissue in response to TSH in the presence of 1 mM theophylline was accompanied by a release of the nucleotide from the tissue into the incubation medium. This cAMP release was almost rectilinearly related to the time of exposure to TSH, and rectilinearly related to the log concentration of TSH in the range 0.1-5 mU/ml.

Oral contraception in diabetic women. A cross-over study on serum and high density lipoprotein (HDL) lipids and diabetes control during progestogen and combined estrogen/progestogen contraception.

Twenty-three young women with insulin-dependent diabetes were randomly allocated to contraceptive treatment with either a progestogen only (Lynestrenol 0.5 mg) (LYN) or a combined oral contraceptive (OC) (ethinyl estradiol 50 micrograms + lynestrenol 2.5 micrograms) (EE + LYN).

Isolation, culture, and preliminary characterization of mucin-producing cells from trachea of the domestic fowl.

Primary cell cultures enriched in mucin-producing cells and basal cells were established from the trachea of the domestic fowl. Epithelial cells were selectively removed from the trachea after incubation in 0.1% pronase/0.