Molecular cloning of grass carp growth hormone receptor and its functional interaction with silver carp growth hormone.

A novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR).

[Molecular mechanisms of plant resistance to cadmium toxicity].

Cadmium (Cd) is a non-essential trace element for plants, and has strong toxicity at low concentrations. It can suppress the elongation growth of plant cell, inhibit oxidative mitochondrial phosphorylation, induce oxidative stress, inhibit the activities of several antioxidative enzymes, affect photosynthesis by inhibiting ferrous reductase or damaging photosynthesis apparatus, and cause the alteration of chromatin and the change of plasma membrane ATPase activity. In response to Cd stress, the cells of cadmium-resistant plant species can produce a number of proteins such as phytochelatins, metallothioneins and stress proteins to detoxify Cd ions, and efficiently repair Cd damage.

[Inhibitory effects of allelopathic rice materials on Echinochloa crus-galli and related field weeds].

By using the modified relay seeding technique, more than 10 allelopathic rice materials were obtained from 500 rice germplasm, and their allelopathic inhibitory effects on weeds were evaluated under field condition. The results showed that under direct-seeded condition, the allelopathic rice materials Gumei 2, Xiayitiao, Jizaoxian, Ganzaoxian and Shangnuo 1 had more obvious inhibitory effects on Echinochloa crus-galli than non-allelopathic rice materials Xiushui 63 and Chunjiang 11, while the allelopathic rice material Milyang 54 had the best inhibitory effect on field weeds. Under field-transplanting condition, the allelopathic rice materials Gumei 2, Qingkun 1, Xiayitiao and Jizaoxian had significant inhibitory effects on Echinochloa crus-galli over non-allelopathic materials Xiushui 63 and Chunjiang 11.

[Interference of allelopathic rice cultivars on barnyardgrass under different water irrigation and rice plant density].

Pot culture experiments were conducted to examine the effects of water irrigation and rice plant density on the interference of allelopathic rice on barnyardgrass (Echinochloa crus-galli). The results showed that under water irrigations, allelopathic rice cultivars Xiayitiao, Gumei 2 and Zhong 156 significantly reduced the plant height of barnyardgrass than non-allelopathic rice cultivars Xiushui 63 and Chunjiang 11. Barnyardgrass plants grew shorter as rice plant density increased.

[The immunopotentiation of human B lymphocyte stimulator C-terminal peptide].

The cDNA of human B lymphocyte stimulator C-terminal peptide (C-BLyS) was amplified by nested PCR from cDNA library of human fetal brain. The expression plasmid pT7450-C-BLyS was constructed and transformed into E. coli BL21 CodonPlus (DE3) RIL which can recognize many rare codons.