Publications by authors named "Gurusamy Ponnudurai"
Article Synopsis
- Tick-borne diseases are a major issue for cattle in tropical and subtropical India, causing significant economic losses for small farmers, particularly in Tamil Nadu.
- A study identified the most common tick species infesting cattle, with Rhipicephalus microplus being the most prevalent, particularly affecting cross-bred cattle more than native breeds.
- The research utilized PCR to assess Theileria annulata infection rates in ticks, revealing that H. anatolicum is the main vector for the disease, highlighting the effectiveness of PCR in detecting low infection levels in ticks.
Aim: The present study was thus undertaken to analyze the genetic diversity of Kilakarsal and Vembur sheep breeds using single-nucleotide polymorphism (SNP) markers within Toll-like receptor (TLR) 3, 5, 6, 9, and 10 genes.
Materials And Methods: Competitive allele-specific polymerase chain reaction (PCR)-based end-point genotyping was performed using real-time PCR to type the SNPs. Allele discrimination module implemented in real-time PCR was utilized to call the genotypes based on fluorescence intensity recorded for each of the two alleles.