Zinc finger proteins facilitate adaptation of a global insect pest to climate change.

Background: Global climate change significantly impacts ecosystems, particularly through temperature fluctuations that affect insect physiology and behavior. As poikilotherms, insect pests such as the globally devastating diamondback moth (DBM), Plutella xylostella, are especially vulnerable to rising temperatures and extreme heat events, necessitating effective adaptive mechanisms.

Results: Here we demonstrate the roles of zinc finger proteins (ZFPs) in mediating thermal adaptability in DBM.

Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest.

The olfactory system of insects plays a pivotal role in multiple, essential activities including feeding, mating, egg laying, and host localization. The capacity of odorant receptors to recognize odor molecules relies on odorant receptor co-receptors forming heterodimers. Here we report the successful engineering a homozygous mutant strain of diamondback moth (Plutella xylostella) in which the odorant receptor co-receptor PxOrco was silenced using CRISPR/Cas9.

Induced plant resistance and its influence on natural enemy use of plant-derived foods.

In response to herbivory, plants employ several inducible defenses to mitigate herbivore damage. These plant-induced responses can trigger subtle changes in plant metabolite composition, altering the profiles of plant-produced exudates such as (extra-) floral nectar and plant guttation. Natural enemies consume these plant-produced exudates, which serve as consistent and nutrient-dense food sources.

Detection and identification of Bogia coconut syndrome phytoplasma from seed-associated tissues and seedlings of coconut (Cocos nucifera) and betel nut (Areca catechu).

Evidence for seed transmission of phytoplasmas has grown in several pathosystems including coconut (Cocos nucifera). Bogia coconut syndrome (BCS) is a disease associated with the lethal yellowing syndrome associated with the presence of 'Candidatus Phytoplasma noviguineense' that affects coconut, betel nut (Areca catechu) and bananas (Musa spp.) in Papua New Guinea.

Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest.

Understanding a population's fitness heterogeneity and genetic basis of thermal adaptation is essential for predicting the responses to global warming. We examined the thermotolerance and genetic adaptation of to exposure to hot temperatures. The population fitness parameters of the hot-acclimated DBM strains varied in the thermal environments.

Developmental Shifts in the Microbiome of a Cosmopolitan Pest: Unraveling the Role of and Dominant Bacteria.

bacteria (phylum Proteobacteria) are ubiquitous intracellular parasites of diverse invertebrates. In insects, coevolution has forged mutualistic associations with species, influencing reproduction, immunity, development, pathogen resistance, and overall fitness. However, the impact of on other microbial associates within the insect microbiome, which are crucial for host fitness, remains less explored.

Harnessing artificial intelligence for analysing the impacts of nectar and pollen feeding in conservation biological control.

Plant-derived foods, such as nectar and pollen, have garnered substantial research attention due to their potential to support natural enemies of pests. This review is a pioneering exploration of the potential for artificial intelligence approaches to provide insights into the factors that drive the success of conservation biological control (CBC). Nectar and pollen were confirmed as key plant food resources for natural enemies.

Field evaluation of electrophysiologically-active dung volatiles as chemical lures for trapping of dung beetles.

Dung beetles are economically important beneficial insects that process dung. To locate this source, they use volatile organic compounds (VOCs). The objectives of the study were to evaluate the attractiveness of ten electrophysiologically-active dung volatiles (phenol, skatole, indole, p-cresol, butanone, butyric acid, eucalyptol, dimethyl sulphide, dimethyl disulphide, and toluene) to dung beetles in the field and to investigate how the composition of volatile blends influences efficacy as lures for use in traps.

Gut bacteria mediated adaptation of diamondback moth, to secondary metabolites of host plants.

In this study, we identify an important role of gut bacteria in mediating the adaptation of diamondback moth (DBM) to plant secondary metabolites. We demonstrate that kaempferol's presence in radish seedlings greatly reduces the fitness of DBM with depleted gut biota. Reinstatement of gut biota, particularly Enterobacter sp.

Too Much Information? A Longitudinal Analysis of Information Overload and Avoidance of Referendum Information Prior to Voting Day.

Previous research has mostly ignored that citizens could experience information overload from a single issue extensively covered in the news. Especially when it comes to issues upon which citizens decide directly in a referendum, overload and avoidance would be problematic from a democracy theory perspective. This study investigates overload and avoidance at the issue level based on a three-wave panel survey on a referendum in Switzerland and finds weak information overload at the aggregate level.

Characterisation of Antennal Sensilla and Electroantennography Responses of the Dung Beetles , and (Coleoptera: Scarabaeoidea) to Dung Volatile Organic Compounds.

Locating sporadically distributed food resources and mate finding are strongly aided by volatile cues for most insects, including dung beetles. However, there is limited information on the olfactory ecology of dung beetles. We conducted a scanning electron microscopy study on the morphology and distribution of the antennal sensilla of three introduced dung beetle species in Australia: (Coleoptera: Geotrupidae), and (Coleoptera: Scarabaeidae).

A very long-chain fatty acid enzyme gene, PxHacd2 affects the temperature adaptability of a cosmopolitan insect by altering epidermal permeability.

Temperature fluctuations pose challenges to poikilotherms, such as insects, especially under climate change conditions. Very long-chain fatty acids (VLCFAs) form important structural components of membranes and epidermal surfaces, so play important roles in adaptation to temperature stress in plants. It has been unclear whether VLCFAs are involved in epidermis formation and thermal resistance in insects.

Continent-wide evidence that landscape context can mediate the effects of local habitats on in-field abundance of pests and natural enemies.

Landscape-scale factors known to influence in-field abundance of pest herbivores and their natural enemies, but little is known about effects that operate through the shorter-range influences exerted by habitats immediately adjacent to crop fields.This study first compared the abundance of brassica insect pests and their natural enemy arthropods in 24 spatially independent brassica vegetable fields across southern Australia. An 'edge effect' index was used to compare the abundance of each taxon in the field center with abundance in areas of the crop adjacent to differing habitats.

DNA metabarcoding of gut contents reveals key habitat and seasonal drivers of trophic networks involving generalist predators in agricultural landscapes.

Background: Understanding the networks of trophic interactions into which generalist predators are embedded is key to assessing their ecological role of in trophic networks and the biological control services they provide. The advent of affordable DNA metabarcoding approaches greatly facilitates quantitative understanding of trophic networks and their response to environmental drivers. Here, we examine how key environmental gradients interact to shape predation by Lycosidae in highly dynamic vegetable growing systems in China.

Contrasting Volatilomes of Livestock Dung Drive Preference of the Dung Beetle (Coleoptera: Scarabaeidae).

Volatile cues can play a significant role in the location and discrimination of food resources by insects. Dung beetles have been reported to discriminate among dung types produced by different species, thereby exhibiting behavioral preferences. However, the role of volatile organic compounds (VOCs) in dung localization and preference remains largely unexplored in dung beetles.

Large-scale genome-wide study reveals climate adaptive variability in a cosmopolitan pest.

Understanding the genetic basis of climatic adaptation is essential for predicting species' responses to climate change. However, intraspecific variation of these responses arising from local adaptation remains ambiguous for most species. Here, we analyze genomic data from diamondback moth (Plutella xylostella) collected from 75 sites spanning six continents to reveal that climate-associated adaptive variation exhibits a roughly latitudinal pattern.

Irreproducibility in searches of scientific literature: A comparative analysis.

Repeatability is the cornerstone of science, and it is particularly important for systematic reviews. However, little is known on how researchers' choice of database, and search platform influence the repeatability of systematic reviews. Here, we aim to unveil how the computer environment and the location where the search was initiated from influence hit results.

Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes.

Sterile male Queensland fruit fly, Bactrocera tryoni (Froggatt), fed as immature adults on the plant compound raspberry ketone (RK), show a reduced attraction to cuelure, a synthetic analogue of RK used as an attractant in Male Annihilation Technique. We hypothesized the reduced attraction of RK-fed adult males to cuelure may be a consequence of altered expression of chemoreception genes. A Y-tube olfactometer assay with RK-fed and RK-unfed sterile B.

Beneficial insects are associated with botanically rich margins with trees on small farms.

Beneficial insect communities on farms are influenced by site- and landscape-level factors, with pollinator and natural enemy populations often associated with semi-natural habitat remnants. They provide ecosystem services essential for all agroecosystems. For smallholders, natural pest regulation may be the only affordable and available option to manage pests.

Molecular gut content analysis indicates the inter- and intra-guild predation patterns of spiders in conventionally managed vegetable fields.

Inter- and intra-guild interactions are important in the coexistence of predators and their prey, especially in highly disturbed vegetable cropping systems with sporadic food resources. Assessing the dietary range of a predator taxon characterized by diverse foraging behavior using conventional approaches, such as visual observation and conventional molecular approaches for prey detection, has serious logistical problems. In this study, we assessed the prey compositions and compare the dietary spectrum of a functionally diverge group of predators-spiders-to characterize their trophic interactions and assess biological control potential in Brassica vegetable fields.

Conservation biological control research is strongly uneven across trophic levels and economic measures.

Conservation biological control suppresses pests by promoting established rather than inoculative or mass released natural enemies. Research in this approach has expanded rapidly this century but uptake remains limited. Why? Most of the 150 peer reviewed papers reporting field experiments include results on natural enemies and/or pests.

Insect pollination is important in a smallholder bean farming system.

Background: Many crops are dependent on pollination by insects. Habitat management in agricultural landscapes can support pollinator services and even augment crop production. Common bean ( L.

Local management and landscape structure determine the assemblage patterns of spiders in vegetable fields.

Both field- and landscape-scale factors can influence the predator communities of agricultural pests, but the relative importance and interactions between these scales are poorly understood. Focusing on spiders, an important taxon for providing biological control, we tested the influence of field- and landscape-scale factors on structuring the spider communities in a highly dynamic brassica agroecosystem. We found that local factors (pesticide-use and crop type) and forested landscape significantly influenced the abundance and species richness of spiders, whilst grassland patches significantly affected the spider species richness.

Fungal Endophyte Communities of Crucifer Crops Are Seasonally Dynamic and Structured by Plant Identity, Plant Tissue and Environmental Factors.

Endophytic fungi are important in diverse plant functions but knowledge of the factors that shape assemblages of these symbionts is lacking. Here, using a culture-dependent approach, we report 4,178 endophytic fungal isolates representing 16 orders isolated from stems, roots and leaves of three cruciferous plant species, Chinese cabbage ( L.), radish ( L.