Publications by authors named "Gurleva G"

Seven Yersinia strains, selected from a number of cultures, not agglutinated by 32 typing sera, were found to have original antigenic features. These strains were given over to the Tarasevich State Research Institute for Standardization and Control of Medical Biological Preparations and registered under O-factor formulae and numbers: Y. kristensenii 165-036, Y.

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It is shown that strains of Vibrio cholerae of serovar O1, biovar eltor, subtype Ogawa, museum strains V. cholerae of serovar O1 and NAG-vibrios (isolated from various sources: sea, river and sewage water, canal water and people) possess identical composition of cell fatty acids with prevailing hexadecenoic, hexadecanoic and octadecenoic acids. Being identical, fatty acid profiles of V.

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Commercial nutrient media and their modifications are assessed, including elective differentiation medium for V. cholerae developed at the Rostov Research Anti-Plague Institute for the isolation of pathogenic vibrios. V.

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Present-day taxonomic status of vibrios pathogenic for humans is discussed, as are the phenotypical characteristics of some species, factors of virulence, and clinical manifestations of diseases caused by these agents. A scheme of isolation and identification of vibrios pathogenic for humans is offered.

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Vibrio cholerae strains, serovar O1, biovar eltor, subtype Ogawa, isolated from different sources, V. cholerae classica and NAG vibrios have identical cell fatty acid composition with the prevalence of hexadecenoic, hexadecanoic and octadecenoic acids. V.

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Group cases of pseudotuberculosis, first registered at health-restoring institutions of the health resort of Gelendzhik (the Krasnodar Territory), are described. The etiology of these cases of the disease was established on the basis of clinico-epidemiological data, specific seroconversion in 66% of the examined patients and the isolation of Yersinia pseudotuberculosis strains from the feces of four patients. The combination of signs observed in the isolated strains, viz.

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92 strains of Yersinia pestis isolated from different natural foci and stored for 3-40 years in the museum of live cultures have been studied. The strains having three typical plasmids, their different combinations, plasmidless strains or the strains carrying nontypical plasmids with the molecular masses 9, 15, 55, 80, 90 and 150 Md were found. The old museum strains are proposed to be used as a source of plasmids for the genetical research.

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Among Yersinia enterocolitica strains of 32 serovars, proposed as typing strains, some strains were found to belong to new species. Y. enterocolitica sensu stricto was represented by 21 serovars in the collection of typing strains.

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Identification of the serological variants according to G. Wauters et al. involves examination of one strain in the agglutination test with 32 sera, and to specify a number of serological variants, investigations with monosera are necessary.

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The aim of the work has been to find the optimal selective medium for the isolation of different serological and biochemical variants of Y. enterocolitica. Endo's, Ploskirev's, Lewin's, and bismuth sulfite media have been under study.

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The serological variants of a number of Y. enterocolitica strains isolated in different regions of the USSR (1,085 strains isolated from humans and animals in the North-West of the RSFSR, 76 strains isolated from humans and animals in the Krasnodar Territory and 114 strains isolated from humans only in the area east of Lake Baikal) were determined. 25 serological variants were registered in these 3 regions of the USSR.

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A phenol fraction, capable of adsorbing cross-reacting antibodies from Y. enterocolitica O9 antiserum without affecting its serological activity against specific antigens, has been obtained from a Brucella strain. The physicochemical and antigenic characteristics of the preparation are presented.

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Seventy-three strains of Y. enterocolitica isolated from patients with acute gastrointestinal diseases and from wild and synathropic rodents in the Krasnodar region were studied for sensitivity to 12 antibiotics. The strains were shown to be sensitive or moderately sensitive to aminoglycosides.

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Sensitivity to 12 antibiotics of Y. enterocolitica and Y. pseudotuberculosis isolated from patients with acute hepatitis in the jaundice form was studied.

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The study of serological properties, specificity and the range of action has revealed affinity between Y. pseudotuberculosis phages (PST, 3M, Kotlyarova, 2344, 2391), some coliphages (T2, T3, T4) and Sh. dysenteriae phage (dd IV).

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The results of the typing of the biochemical and serological variants of Yersinia enterocolitica strains from the USSR (10 strains) and other countries (32 strains) are presented. Most of these strains, belonging to biovars 1 and 5, could be determined with the use of the antigenic scheme which was proposed by Knapp only for biovars 2, 3 and 4. The strains isolated in Leningrad and in the Far East were found to belong to serovars 6,30; 7,8; 12,25 and 5B according to the scheme proposed by Wauters; of these, the last one corresponds to serovar IV in Knapp's scheme.

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227 out of 228 strains of pseudotuberculosis microbes studied in vitro proved to be sensitive to tetracyclines. The MIC of tetracycline and morphocycline ranged within 0.25--25 gamma/ml.

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It was found that 229 out of 230 strains of pseudotuberculosis microbes tested were sensitive to aminoglycosides, such as streptomycin, monomycin, neomycin, kanamycin and gentamicin. The MIC did not exceed 50 gamma/ml. One strain of J.

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