[Reposterior individualized three-column osteotomy for tuberculous spinal angular kyphosis deformity].

Objective: To investigate the efficacy of posterior three-columns osteotomy in the treatment of severe tuberculous angular kyphosis.

Methods: Total of 33 patients with severe tuberculous angular kyphosis were treated with posterior three-columns osteotomy from January 2006 to January 2019 including 24 males and 9 females with an average age of (40.6±23.

One-step genome editing of elite crop germplasm during haploid induction.

Genome editing using CRISPR-Cas9 works efficiently in plant cells, but delivery of genome-editing machinery into the vast majority of crop varieties is not possible using established methods. We co-opted the aberrant reproductive process of haploid induction (HI) to induce edits in nascent seeds of diverse monocot and dicot species. Our method, named HI-Edit, enables direct genomic modification of commercial crop varieties.

Characterization of a potato activation-tagged mutant, nikku, and its partial revertant.

A potato mutant with a strong stress-response phenotype, and a partial mutant revertant, were characterized. Gene expression patterns and DNA cytosine methylation varied between these and wild-type, indicating a role for DNA cytosine methylation changes in the gene expression and visible phenotypes. Morphological and molecular studies were conducted to compare potato cv.

Characterization and RNA-seq analysis of underperformer, an activation-tagged potato mutant.

The potato cv. Bintje and a Bintje activation-tagged mutant, underperformer (up) were compared. Mutant up plants grown in vitro were dwarf, with abundant axillary shoot growth, greater tuber yield, altered tuber traits and early senescence compared to wild type.