Publications by authors named "Guozhong Zhang"

Objective: The aim of this research was to investigate the specific regulatory role of miR-6760-5p in angiogenesis in moyamoya disease.

Methods: HUVECs were transfected with miR-6760-5p inhibitor and mimics fragments, then subjected to assays for cell proliferation, migration, and tube formation. Subsequently, downstream target genes of miR-6760-5p were predicted and the protein expression levels of these genes were evaluated.

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  • * Forensic pathology often overlooks the connection between brain injuries and cardiac health in death cases shortly after such injuries, particularly when heart disease is already present.
  • * This article examines recent research on cerebrocardiac syndrome and highlights its importance for forensic medicine, aiming to improve practices in this area.
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  • * A study sequenced 66 strains of SFTSV from Hangzhou, China, revealing five genotype groups, with genotype A being the most prevalent and genotype E linked to higher fatality rates.
  • * The research identified several novel genetic reassortants and significant genetic diversity, highlighting the need for enhanced surveillance in Hangzhou to monitor SFTSV evolution.
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  • MHC-I (Major Histocompatibility Complex Class I) is essential for immune responses against viral infections, involving both CD8 T cells and natural killer (NK) cells; understanding how viruses like PEDV manipulate MHC-I is crucial for developing defenses.
  • This study shows that while PEDV infection boosts the production of NLRC5 (a key regulator for MHC-I transcription), it paradoxically leads to a decrease in MHC-I protein levels due to the interference of PEDV’s nonstructural protein 1 (nsp1), which inhibits NLRC5's effect.
  • The research uncovers a mechanism of immune evasion where PEDV-infected cells appear similar to healthy cells, allowing the virus to escape
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Skeletal muscle contusion (SMC) is common in daily life and clinical practice, but the molecular mechanisms underlying SMC healing are unclear. Ferroptosis, a regulated cell death type, has gained attention recently. We observed iron overload in skeletal muscle following contusion through HE and Perls staining.

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Long interspersed nuclear element‑1 (L1) is highly expressed in the early embryos of humans, rodents and fish. To investigate the molecular mechanisms underlying high expression of L1 during early embryonic development, a C1‑open reading frame (ORF)2 vector was constructed in which ORF2 of human L1 (L1‑ORF2) was inserted into a pEGFP‑C1 plasmid. C1‑ORF2 vector was injected into early zebrafish embryos (EZEs) to observe expression of EGFP reporter protein by fluorescence microscopy.

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  • - Sepsis-related organ damage, particularly sepsis-associated liver injury (SALI), is a significant challenge in intensive care, often resulting in poor patient outcomes due to complex physiological issues.
  • - The study investigates the effectiveness of maraviroc (MVC), a CCR5 inhibitor, in treating SALI using a cecal ligation and puncture (CLP) model, where MVC was injected post-surgery, leading to significant improvements in liver injury.
  • - Results indicate that MVC reduces liver inflammation and cell apoptosis by inhibiting specific inflammatory signaling pathways (NF-κB and MAPK), with these effects reversed when a JNK agonist was used, highlighting MVC's potential in managing SALI.
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This article explores the important functions of transfer RNA and - transfer RNA derived small RNAs (tsRNAs) in cellular processes and disease pathogenesis, with a particular emphasis on their involvement in cerebrovascular disorders. It discusses the biogenesis and structure of tsRNAs, including types such as tRNA halves and tRNA-derived fragments, and their functional significance in gene regulation, stress response, and cell signaling pathways. The importance of tsRNAs in neurodegenerative diseases, cancer, and cardiovascular diseases has already been highlighted, while their role in cerebrovascular diseases is in early phase of exploration.

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Porcine epidemic diarrhea virus (PEDV) is an enteropathogenic coronavirus that causes substantial economic loss to the global pig industry. The emergence of PEDV variants has increased the need for new vaccines, as commercial vaccines confer inferior protection against currently circulating strains. It is well established that the induction of mucosal immunity is crucial for PEDV vaccines to provide better protection against PEDV infection.

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The effect of ball milling on the physicochemical properties and gut microbiota regulation of Poria cocos pachyman (PAC) was investigated. Ball milling reduced the particle size of PAC from 102 μm to 25.19 μm after 12 h, resulting in increasing particle uniformity.

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Like all coronaviruses, infectious bronchitis virus, the causative agent of infectious bronchitis in chickens, exhibits a high mutation rate. Adaptive mutations that arise during the production of live attenuated vaccines against IBV often decrease virulence. The specific impact of these mutations on viral pathogenicity, however, has not been fully elucidated.

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Unlabelled: Viruses have evolved a range of strategies to utilize or manipulate the host's cellular translational machinery for efficient infection, although the mechanisms by which infectious bronchitis virus (IBV) manipulates the host translation machinery remain unclear. In this study, we firstly demonstrate that IBV infection causes host shutoff, although viral protein synthesis is not affected. We then screened 23 viral proteins, and identified that more than one viral protein is responsible for IBV-induced host shutoff, the inhibitory effects of proteins Nsp15 were particularly pronounced.

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Inactivated and live attenuated vaccines are the mainstays of preventing viral poultry diseases. However, the development of recombinant DNA technology in recent years has enabled the generation of recombinant virus vector vaccines, which have the advantages of preventing multiple diseases simultaneously and simplifying the vaccination schedule. More importantly, some can induce a protective immune response in the presence of maternal antibodies and offer long-term immune protection.

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Newcastle disease virus (NDV) is an avian pathogen with an unsegmented negative-strand RNA genome that belongs to the Paramyxoviridae family. While primarily pathogenic in birds, NDV presents no threat to human health, rendering it a safe candidate for various biomedical applications. Extensive research has highlighted the potential of NDV as a vector for vaccine development and gene therapy, owing to its transcriptional modularity, low recombination rate, and lack of a DNA phase during replication.

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  • Stress can cause significant damage to liver cells, highlighting a notable lack of research in this area, which this study aimed to address.* -
  • The researchers developed a mouse model of restraint stress and used various advanced techniques to analyze liver function and cell damage, finding that oxidative stress plays a key role.* -
  • The findings suggest that the methylation of the GSTP1 gene contributes to liver damage during stress, implicating a pathway (TF/Nrf2) linked to a specific type of cell death called ferroptosis.*
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Infectious bronchitis virus (IBV) is a coronavirus that infects chickens, which exhibits a broad tropism for epithelial cells, infecting the tracheal mucosal epithelium, intestinal mucosal epithelium, and renal tubular epithelial cells. Utilizing single-cell RNA sequencing (scRNA-seq), we systematically examined cells in renal, bursal, and tracheal tissues following IBV infection and identified tissue-specific molecular markers expressed in distinct cell types. We evaluated the expression of viral RNA in diverse cellular populations and subsequently ascertained that distal tubules and collecting ducts within the kidney, bursal mucosal epithelial cells, and follicle-associated epithelial cells exhibit susceptibility to IBV infection through immunofluorescence.

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Tumor-specific T cells are crucial in anti-tumor immunity and act as targets for cancer immunotherapies. However, these cells are numerically scarce and functionally exhausted in the tumor microenvironment (TME), leading to inefficacious immunotherapies in most patients with cancer. By contrast, emerging evidence suggested that tumor-irrelevant bystander T (T) cells are abundant and preserve functional memory properties in the TME.

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Introduction: is a Gram-negative, flagellated, motile bacillus within the . The case reports of clinical infections shed light on the importance of this organism as an emerging opportunistic pathogen. The genus , which often be misidentified with , is also an important clinically relevant member of the .

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  • Coronary artery spasm (CAS) is a significant factor in ischemic heart diseases and can lead to life-threatening heart rhythm issues, yet its molecular mechanisms and specific diagnostic markers remain unclear.
  • This study developed a rat model of CAS and a human smooth muscle cell contraction model to investigate the role of the protein kinase C (PKC) signaling pathway in regulating CAS through the CPI-17/MLC2 mechanism.
  • Findings revealed that PKCα translocates in CAS-affected coronary arteries, activating CPI-17/MLC2 signaling, and showed potential diagnostic markers in human tissue samples from CAS-suspected deaths.
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  • Stress negatively impacts liver function by causing mitochondrial damage, potentially through the action of Ceramide synthase 6 (CerS6)-derived C16:0 ceramide.
  • In experiments using rats and hepatocyte models, stress led to elevated production of C16:0 ceramide and activation of certain cellular pathways that contribute to mitochondrial damage.
  • Knocking down CerS6 in liver cells reduced the stress-related increase in C16:0 ceramide, indicating its significant role in stress-induced liver injury.
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  • * In experiments with stressed rats, it was found that norepinephrine (a stress hormone) increased, causing cell swelling and higher levels of a protein called AQP4 in astrocytes, suggesting that stress has a damaging effect.
  • * The research highlights a specific pathway (p38 MAPK) activated by norepinephrine that leads to these changes, which could help develop new treatments for stress-related conditions and neuropsychiatric disorders.
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Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne infectious disease with sporadic occurrence and high mortality. Herein, we report an example of the in-hospital transmission of SFTS virus (SFTSV) infections with familial and nosocomial clustering in Zhejiang Province, eastern China, from March to April 2023. The epidemiological investigation and genomic analysis revealed that at least eight suspected cases of SFTS occurred in this cluster, including one death and one asymptomatic case.

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Gut microbiota-derived metabolites are important for the replication and pathogenesis of many viruses. However, the roles of bacterial metabolites in swine enteric coronavirus (SECoV) infection remain poorly understood. Recent studies show that SECoVs infection significantly alters the composition of short-chain fatty acids (SCFAs)-producing gut microbiota.

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Interdisciplinary collaboration is an emerging imperative in music education, but current understanding of global practices remains limited. Prior studies have focused narrowly on specific programs within limited geographic areas. However, there is minimal quantitative mapping of initiatives across institutions and regions.

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