Publications by authors named "Guozhong Chen"
Machine learning and network analysis with focus on the biofilm in .
Comput Struct Biotechnol J
December 2024
Article Synopsis
- Research has advanced understanding of biofilm formation by utilizing high-throughput sequencing data, particularly transcriptomic data, to analyze the genetic networks involved.
- Machine learning and differential expression analysis have been employed to identify key genes and pathways that influence the transition of bacteria from a free-living state to a biofilm state.
- An online database, SAdb, was created to provide easy access to integrated data on gene annotations, transcriptomics, and proteomics, aiming to support researchers studying biofilms and their significance in bacterial behavior and resistance.
BBSdb, an open resource for bacterial biofilm-associated proteins.
Front Cell Infect Microbiol
August 2024
Article Synopsis
- Bacterial biofilms are complex communities of microorganisms enclosed in a protective matrix, and recent research has revealed numerous proteins associated with these biofilms.
- The BBSdb was created as a comprehensive open resource to consolidate various biofilm-related data, including extensive datasets on gene and protein expressions from multiple bacterial species.
- This database, available online, aims to aid researchers in the field of microbiology by providing easy access to vital information on biofilm-associated proteins, which are crucial for understanding bacterial growth and resistance.
Article Synopsis
- The study investigates the potential of using pre-operative ultrasonographic gastric measurements to predict postoperative nausea and vomiting (PONV) in patients undergoing elective gynecological laparoscopic surgery.
- The results indicate that a greater cross-sectional area of the inner side of the muscularis propria (CSA-ISMP) is associated with higher PONV risk in the first 6 hours post-surgery, while a thinner muscularis propria (TMP) offers protection against PONV in the 6-24 hour window.
- The findings suggest that these gastric morphological parameters could enhance pre-operative assessments for predicting PONV, improving patient management.
Article Synopsis
- Portal vein gas accumulation and intestinal pneumatosis can signal high mortality risk in intestinal ischemic necrosis, although benign cases are on the rise due to advanced imaging techniques.
- A male patient with organophosphorus pesticide poisoning presented with severe symptoms and was treated with mechanical ventilation and renal support.
- Despite the serious implications of his condition, abdominal imaging revealed signs of potential benignity, suggesting that conservative treatment may be considered instead of immediate surgery in some cases.
Article Synopsis
- Most vaccinees and those recovered from COVID-19 develop strong immunity against SARS-CoV-2, which helps prevent infections and reduce symptoms, yet the emergence of new variants like Omicron still raises global health concerns.
- Surveillance of long-term cohorts shows a rise in breakthrough infections related to immune evasion by Omicron variants, with indications of reduced neutralization capacity over time.
- The study suggests that sequential infections with specific variants can diminish immune imprinting from inactivated vaccines, underscoring the need for updated booster vaccinations to tackle reinfection risks from variants like Omicron JN.1.
A bibliometric analysis of non-coding RNA studies in acute pancreatitis.
Medicine (Baltimore)
March 2024
Article Synopsis
- The study focuses on the role of non-coding RNA (ncRNA) in acute pancreatitis, highlighting its importance in disease onset, progression, and therapy.
- A bibliometric analysis was conducted using data from 2000-2023, revealing 563 publications mainly from China and identifying key research themes such as inflammation, microRNA expression in pancreatic cancer, and ncRNA as diagnostic markers.
- The findings indicate that ncRNA research is well-established and provide insights for future studies, suggesting potential research directions based on emerging hotspots like injury pathways and extracellular vesicles.
Article Synopsis
- The research aimed to investigate the potential link between heavy metal exposure and the occurrence of kidney stones in American adults.
- Data from 29,201 individuals were analyzed, resulting in the inclusion of 8,518 participants, revealing that higher levels of urinary cobalt (U-Co) and cadmium (U-Cd) were associated with increased rates of kidney stones.
- The findings suggest that elevated urinary cobalt levels are significantly correlated with a higher risk of developing kidney stones in the U.S. population.
Article Synopsis
- Safer and more effective treatments for acute pancreatitis (AP) are needed, and Qingjie Huagong decoction (QJHGD) has shown promising clinical effects but its mechanisms were unclear.
- The study aimed to explore how QJHGD influences AP both in cell cultures and in mouse models, using various biochemical and molecular techniques to analyze its effects.
- Results indicated that QJHGD enhances cell viability and reduces pancreatic injury by regulating a specific pathway involving circHipk3, miR-193a-5p, and NLRP3, which highlights a new mechanism for its therapeutic action against AP.
Article Synopsis
- Scientists found a new virus called Zoothera dauma adenovirus (ZdAdV) in a wild bird species known as the Scaly thrush.
- The virus is unique and belongs to a family called Atadenovirus, with a genome that has 28 predicted genes.
- The discovery may help us understand how viruses can share genes between different species, which could be important for studying diseases.
Article Synopsis
- The study evaluated the effectiveness and safety of adamgammadex compared to sugammadex for reversing deep neuromuscular blockade caused by rocuronium in 80 patients undergoing elective surgery.
- Both drugs showed similar outcomes in recovery times and success rates, with adamgammadex 9 mg/kg allowing recovery in an average of 2.9 minutes.
- Adamgammadex demonstrated better tolerance and a lower incidence of adverse events than sugammadex, suggesting it could be a promising alternative for reversing neuromuscular blockade.
Article Synopsis
- The study examines the effectiveness of arterial spin labeled (ASL) and blood oxygen level dependent (BOLD) imaging in assessing kidney function after transplantation in 135 patients.
- The findings indicate that ASL and BOLD parameters are connected to kidney function damage and transplant health, with a prediction model showing a high accuracy for renal allograft function using these imaging techniques.
- The results also reveal correlations between patient demographics, imaging parameters, and kidney function, suggesting that ASL and BOLD may be useful tools for tracking transplant outcomes.
Article Synopsis
- The study focuses on how certain bacteria adapt to their host’s gut environment by changing their gene expression, particularly in fully colonized adult mice.
- Through the innovative use of coated-magnetic chitin beads (vcMCB), the researchers successfully extracted high-quality RNA, allowing for a detailed analysis of gene expression differences between bacteria from adult mice and those from infant models.
- The findings highlight a specific bacterial strain's ability to survive low pH and formic acid by altering its gene expression, offering new insights into the relationship between bacteria and gut microbiota, which can inform future research on other pathogenic bacteria.
Article Synopsis
- YiShen HuoXue decoction (YSHXD) is a traditional formulation used for treating renal fibrosis (RF), and this study investigates its therapeutic effects and underlying mechanisms.
- The research employed network pharmacology and machine-learning to identify key components and targets, followed by experimental validation using HK-2 cells and a rat model induced by unilateral ureteral ligation (UUO).
- Key active compounds like quercetin and kaempferol were found to act on target proteins such as IL-6 and TNF, with YSHXD showing anti-inflammatory effects by inhibiting specific pathways involved in cell death related to RF.
Article Synopsis
- The study aimed to identify the key targets and mechanisms of Dahuang-Danshen (DH-DS) in treating acute pancreatitis (AP) using network pharmacology and animal experiments.
- Sixty-seven common targets were found, with MAPK3, JAK2, STAT3, and FOS highlighted as core targets, while gene ontology and pathway analyses identified cellular response and Th17 cell differentiation as main mechanisms.
- Animal tests confirmed that DH-DS effectively reduced inflammation and protected pancreatic tissue by inhibiting specific inflammatory markers and regulating Th17 cell differentiation.
Article Synopsis
- GRK2 is implicated in the activation of lung fibroblasts by TGF-β1, which may lead to pulmonary fibrosis.
- Paroxetine (PRXT), a selective GRK2 inhibitor used for anxiety and depression, shows potential in improving lung function and survival in mice with bleomycin-induced pulmonary fibrosis.
- PRXT also reduces inflammation and collagen deposition in lung tissues while inhibiting GRK2 and Smad3 expression, highlighting its therapeutic potential for treating pulmonary fibrosis.
Article Synopsis
- Epidemiological research shows low resistance to nitrofuran in clinical isolates, making it promising for treating multidrug-resistant bacteria, necessitating an understanding of resistance mechanisms.
- A study identified the MarR protein VnrR as key in defending against the nitrofuran derivative furazolidone (FZ), which responds to its metabolites and promotes gene activation linked to FZ degradation.
- VnrR aids in FZ metabolism through a reactive oxygen species scavenging pathway, enhancing our comprehension of antibiotic resistance and informing strategies for managing multidrug-resistant infections.
Article Synopsis
- Acute lung injury (ALI) is a serious condition primarily caused by excessive inflammation, often modeled in animals using lipopolysaccharide (LPS).
- Researchers used MyD88 knockdown heterozygous mice to study how partially reducing the MyD88 gene affects LPS-induced ALI, finding that it reduced lung damage and fibrosis.
- The study concluded that MyD88 plays a crucial role in LPS-induced inflammation and lung injury, as its incomplete knockdown can alleviate severe symptoms by inhibiting certain inflammatory pathways.
Article Synopsis
- Using black soldier fly larvae (BSFLs) for food waste treatment is a promising eco-friendly approach.
- The study investigated how varying nutritional compositions (standard, high-protein, high-fat, and high-starch diets) impacted the intestinal microbiota and digestive enzymes of BSFLs.
- Results showed that high-protein diets reduced microbial diversity and enzyme activity, leading to higher mortality rates, while high-fat diets improved growth, survival, and microbiota diversity despite lower enzyme activity.
Article Synopsis
- A significant study integrated transcriptome data from both RNA-seq and microarray platforms for Vibrio cholerae, marking the first effort to combine these two types of data.
- By analyzing the integrated data, researchers identified key gene expressions and functional modules related to stress responses, gene manipulation, and metabolic pathways.
- The study also revealed novel protein interactions and highlighted similarities between clinical samples and experimental conditions, providing valuable insights into V. cholerae's pathogenic mechanisms.
Spatial distribution of airborne bacterial communities in caged poultry houses.
J Air Waste Manag Assoc
May 2023
Article Synopsis
- The study investigated the distribution and diversity of bacterial aerosols in broiler houses, revealing variations in concentrations related to different locations within the house.
- Significant alpha diversity was found at the entry of the broiler house compared to the middle and rear, suggesting a richer microbial community near the entrance.
- The research highlighted the connection between airborne bacteria in poultry environments and their impact on animal health and production, offering fresh insights for future studies.
Article Synopsis
- V. cholerae develops complex mechanisms to handle host stress, leading to the emergence of nonmotile mutants that enhance its colonization in mice.
- Mutations primarily occur in flagellar regulator genes like flrA, flrC, and rpoN, rather than in traditional genes related to DNA repair or scavenging systems.
- The cyclin gene dps plays a crucial role, with its deletion resulting in a significant increase in mutation rates, suggesting it influences the formation of nonmotile mutants as a strategy for better adaptation in the host environment.
Article Synopsis
- Atopic dermatitis (AD) is a long-lasting skin condition influenced by an imbalance in skin microbes, and the role of skin bacteria in managing AD is being explored.
- Staphylococcus epidermidis-derived extracellular vesicles (SE-EVs) were found to lower inflammatory gene expression and promote skin cell growth and movement, offering protective benefits against bacteria like S. aureus.
- Applying SE-EVs in a mouse model of AD reduced skin inflammation and immune responses, suggesting that they could be a potential treatment for AD.
Article Synopsis
- Researchers sequenced the complete genome of a new aviadenovirus from a Eurasian scops owl in China, revealing a 40,239-bp genome with 30 predicted protein-coding genes.
- The new virus has a highly divergent genetic structure, with encoded proteins showing only 55% identity to other adenoviruses and 62% identity to a previously reported owl adenovirus from Japan.
- This study highlights the diversity of aviadenoviruses in wild birds and proposes that the newly discovered owl virus and the Japanese variant should be classified as two new species within the genus Aviadenovirus.