Cleavable energy transfer labeled oligonucleotide probe for enhanced isothermal amplification detection and nano digital chip-based readout.

Quantitative analysis of human papillomavirus (HPV)-infected cervical cancer is essential for early diagnosis and timely treatment of cervical cancer. Here, we introduce a novel energy transfer-labeled oligonucleotide probe to enhance the loop-mediated isothermal amplification (LAMP) assay for highly sensitive and specific detection of HPV 16. Conducted as a single-step assay within a digital nanofluidic chip featuring numerous reaction reservoirs, our method facilitates target amplification under isothermal conditions.

Dual-task vision transformer for rapid and accurate intracerebral hemorrhage CT image classification.

Intracerebral hemorrhage (ICH) is a severe and sudden medical condition caused by the rupture of blood vessels in the brain, leading to permanent damage to brain tissue and often resulting in functional disabilities or death in patients. Diagnosis and analysis of ICH typically rely on brain CT imaging. Given the urgency of ICH conditions, early treatment is crucial, necessitating rapid analysis of CT images to formulate tailored treatment plans.

[Isongifolene Improves Crohn's Disease-Like Colitis in Mice by Reducing Apoptosis of Intestinal Epithelial Cells].

Objective: To investigate the effect and molecular mechanism of isolongifolene (ISO) on the apoptosis of intestinal epithelial cells and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced Crohn's disease (CD)-like colitis in mice.

Methods: In the animal experiments, mice were randomly assigned to the wild type (WT) group, TNBS group and TNBS+ISO group, with 8 mice in each group. Colitis models of mice were established in the TNBS group and the TNBS+ISO group by rectal injection of TNBS.

The Use of Imaging to Quantify the Impact of Seed Aging on Lettuce Seed Germination and Seedling Vigor.

The decline in seed quality over time due to natural aging or mishandling requires assessing seed vigor for resilience in adverse conditions. Accelerated aging (AA) methods simulate seed deterioration by subjecting seeds to high temperatures and humidity. Saturated salt accelerated aging (SSAA) is an AA method adopted for small seeds like lettuce ().

Automated Imaging to Evaluate the Exogenous Gibberellin (Ga) Impact on Seedlings from Salt-Stressed Lettuce Seeds.

Salinity stress is a common challenge in plant growth, impacting seed quality, germination, and general plant health. Sodium chloride (NaCl) ions disrupt membranes, causing ion leakage and reducing seed viability. Gibberellic acid (GA) treatments have been found to promote germination and mitigate salinity stress on germination and plant growth.

Low-Cost Imaging to Quantify Germination Rate and Seedling Vigor across Lettuce Cultivars.

The survival and growth of young plants hinge on various factors, such as seed quality and environmental conditions. Assessing seedling potential/vigor for a robust crop yield is crucial but often resource-intensive. This study explores cost-effective imaging techniques for rapid evaluation of seedling vigor, offering a practical solution to a common problem in agricultural research.

Molecular epidemiology and clinical characteristics of respiratory syncytial virus in hospitalized children during winter 2021-2022 in Bengbu, China.

Objective: This study aimed to study the molecular epidemiology and clinical characteristics of respiratory syncytial virus (RSV) infection from hospitalized children with ARTI in Bengbu.

Methods: One hundred twenty-four nasopharyngeal swab specimens and clinical data from children with ARTI cases were collected in Bengbu, China, during winter 2021-2022. The samples were detected by qPCR of 13 respiratory viruses.

Interindividual distances and orientations of laying hens under 8 stocking densities measured by integrative deep learning techniques.

Interindividual distances and orientations of laying hens provide quantitative measures to calculate and optimize space allocations for bird flocks. However, these metrics were often measured manually and have not been examined for different stocking densities of laying hens. The objectives of this study were to 1) integrate and develop several deep learning techniques to detect interindividual distances and orientations of laying hens; and 2) examine the 2 metrics under 8 stocking densities via the developed techniques.

Star map matching method for optical circular rotation imaging based on graph neural networks.

This paper focuses on a dynamic star image acquisition and matching method for space situational awareness, which can quickly search for widely distributed resident space objects. First, the optical circular rotation imaging method performed by a single space camera is proposed to obtain a series of star images. And then, the image matching method based on graph neural networks is proposed for generating a wide observation star image.

Saikosaponin D attenuates inflammatory response and cell apoptosis of lipopolysaccharide-induced lung epithelial cells.

Background: Acute lung injury (ALI) is a prevalent complication of sepsis with high mortality rate. Saikosaponin D (SSD) is a triterpenoid saponin that has been reported to alleviate sepsis-triggered renal injury in mice. Nonetheless, the therapeutic effect of SSD on sepsis-evoked ALI is unclarified.

Superhydrophobic Rotation-Chip for Computer-Vision Identification of Drug-Resistant Bacteria.

The transport, distribution, and mixing of microfluidics often require additional instruments, such as pumps and valves, which are not feasible when operated in point-of-care (POC) settings. Here, we present a simple microfluidic pathogen detection system known as Rotation-Chip that transfers the reagents between wells by manually rotating two concentric layers without using external instruments. The Rotation-Chip is fabricated by a simple computer numerical control (CNC) machining process and is capable of carrying out 60 multiplexed reactions with a simple 30 or 60° rotation.

An End-to-End Steel Surface Classification Approach Based on EDCGAN and MobileNet V2.

In the production process of steel products, it is very important to find defects, which can not only reduce the failure rate of industrial production but also can reduce economic losses. All deep learning-based methods need many labeled samples for training. However, in the industrial field, there is a lack of sufficient training samples, especially in steel surface defects.

Computer vision enabled funnel adapted sensing tube (FAST) for power-free and pipette-free nucleic acid detection.

A simple, portable, and low-cost microfluidic system-funnel adapted sensing tube (FAST) is developed as an integrated, power-free, and pipette-free biosensor for viral nucleic acids. This FAST chip consists of four reaction chambers separated by carbon fiber rods, and the reagents in each chamber are transferred and mixed by manually removing the rods. Rather than using electrical heaters, only a hand warmer pouch is used for an isothermal recombinase polymerase amplification (RPA) and CRISPR-Cas12a reaction.

Berberine attenuates sepsis-induced cardiac dysfunction by upregulating the Akt/eNOS pathway in mice.

The present study aimed to investigate the cardioprotective role of berberine in sepsis-induced cardiac dysfunction and consider the underlying mechanisms. C57BL/6J mice were randomized into four groups, namely, Control, lipopolysaccharide (LPS), LPS + berberine and LPS + N-nitro-L-arginine methyl ester (L-NAME) + berberine. A single dose (10 mg/kg body weight) of LPS was intraperitoneally administered to mice to induce cardiac dysfunction, whereas the Control group was administered with an equivalent volume of saline.

Simultaneous Direct Depth Estimation and Synthesis Stereo for Single Image Plant Root Reconstruction.

Plant roots are the main conduit to its interaction with the physical and biological environment. A 3D root system architecture can provide fundamental and applied knowledge of a plant's ability to thrive, but the construction of 3D structures for thin and complicated plant roots is challenging. Existing methods such as structure-from-motion and shape-from-silhouette require multiple images, as input, under a complicated optimization process, which is usually not convenient in fieldwork.

Hyperspectral face recognition based on sparse spectral attention deep neural networks.

Inspired by the robust capability and outstanding performance of convolutional neural networks (CNN) in image classification tasks, CNN-based hyperspectral face recognition methods are worthy of further exploration. However, hyperspectral imaging poses new challenges including high data dimensionality and interference between bands on spectral dimension. High data dimensionality can result in high computational costs.

The aberrant expressions of MACC1, ZEB1, and KLF4 in hepatocellular carcinoma and their clinical significance.

Background And Objective: Metastasis-associated in colon cancer-1 (MACC1) is involved in the progression and metastasis of various cancers. Zinc finger E-box-binding homeobox 1 (ZEB1) is a key transcriptional factor of the epithelial-mesenchymal transition (EMT) that is involved in the migration and invasion of cancer cells. Kruppel-like factor 4 (KLF4) is a tumor suppressor that can inhibit tumor cell proliferation, migration, and metastasis.

Value of CagA, HER2, ALDH1, and KiSS-1 in predicting metastasis and prognosis for gastric adenocarcinoma.

Background: Cytotoxin-associated gene A (CagA), is able to translocate into gastric epithelial cells. Human epidermal growth factor receptor 2 (also named as HER2, is a proto-oncogene which can encode a transmembrane receptor), Aldehyde dehydrogenase 1 (ALDH1, a biomarker of cancer stem cells), and KiSS-1 (a suppressor gene of cancer metastasis) are all valuably predictive biomarkers for various human cancers. The purpose of this study is to investigate the associations among CagA, HER2, ALDH1, and KiSS-1 in gastric adenocarcinoma (GAC), and their respective associations with clinical characteristics and survival in GAC.

Expression of ORAOV1, CD133 and WWOX correlate with metastasis and prognosis in gastric adenocarcinoma.

Background: Oral cancer overexpressed 1 (ORAOV1) which is a novel candidate oncogene is a useful biomarker of metastasis and prognosis in various cancers. CD133 which is a biomarker of cancer stem cells is overexpressed in many cancers and promotes cancer cells growth and metastasis. WW domain-containing oxidoreductase (WWOX) which is a suppressor gene of tumor can inhibit proliferation and promote apoptosis in various cancers.

Pharmacokinetic/Pharmacodynamic Analysis of Meropenem for the Treatment of Nosocomial Pneumonia in Intracerebral Hemorrhage Patients by Monte Carlo Simulation.

Background: Nosocomial pneumonia (NP) is a frequent complication among patients with intracerebral hemorrhage (ICH). However, there are currently no pharmacokinetic (PK) and pharmacodynamic (PD) data to guide meropenem dosing in these patients.

Objective: To investigate the PK/PD properties of meropenem in these patients and whether the usual dosing regimens of meropenem (2-hour infusion, 1 g, every 8 hours) was suitable.

Antifreeze poisoning: A case report.

The current study reported the case of a 35-year-old male that presented with antifreeze poisoning. The clinical manifestations, laboratory investigations and treatments were analyzed, and the obtained results were compared with those in previous reports. Subsequent to consuming antifreeze, the patient mainly presented nausea and agitation, without disturbance of consciousness.

The expression of metastasis-associated in colon cancer-1 and KAI1 in gastric adenocarcinoma and their clinical significance.

Background: The most common reason for malignant tumor treatment failure is recurrence and metastasis. Metastasis-associated in colon cancer-1 (MACC1) was originally identified as a metastatic and prognostic biomarker for colon cancer and later other solid tumors. Kangai 1 (KAI1), a marker of suppressor of metastasis, is also associated with metastasis and poor prognosis in many tumors.

[Expressions of CD133 and CD44 in gastric adenocarcinoma and their relationship with E-cadherin expression].

Objective: To explore the expression of CD133 and CD44 in the primary gastric adenocarcinoma (GAC) and their relationship with the expression of E-cadherin.

Methods: The expressions of CD133, CD44, and E-cadherin was examined by immunohistochemistry in 145 specimens of GAC tissues and 30 specimens of normal gastric tissues.

Results: The positivity rates of CD133, CD44, and E-cadherin in normal gastric tissues were 10.

Tissue engineering scaffold material of porous nanohydroxyapatite/polyamide 66.

The aim of the study was to investigate a porous nanohydroxyapatite/polyamide 66 (n-HA/PA66) scaffold material that was implanted into muscle and tibiae of 16 New Zealand white rabbits to evaluate the biocompatibility and osteogenesis and osteoinductivity of the materials in vivo. The samples were harvested at 2, 4, 12 and 26 weeks respectively, and subjected to histological analysis. At 2 weeks, the experiment showed that osteogenesis was detected in porous n-HA/PA66 composite and the density of new bone formation was similar to the surrounding host bone at 12 weeks.