LincRNA01703 Facilitates CD81 Exosome Secretion to Inhibit Lung Adenocarcinoma Metastasis via the Rab27a/SYTL1/CD81 Complex.

Metastasis, a major cause of cancer-related mortality worldwide, frequently occurs early in the diagnosis of lung adenocarcinoma (LUAD). However, the precise molecular mechanisms governing the aggressive metastatic behavior of LUAD remain incompletely understood. In this study, we present compelling evidence indicating that the long noncoding RNA linc01703 is significantly downregulated in metastatic lung cancer cells.

Multi-omics analysis reveals PUS1 triggered malignancy and correlated with immune infiltrates in NSCLC.

Non-small cell lung cancer (NSCLC) is the main pathological type of lung cancer. In this study, multi-omics analysis revealed a significant increase of pseudouridine synthase 1 (PUS1) in NSCLC and the high expression of PUS1 was associated with shorter OS (Overall Survival), PFS (Progression Free Survival), and PPS (Post Progression Survival) of NSCLC patients. Clinical subgroup analysis showed that PUS1 may be involved in the occurrence and development of NSCLC.

Intermittent water supply and self-rated health in rural China's karst region.

Introduction: With rapid economic and social development, surging demand for domestic water, and the increasing shortage of water resources, the applications of intermittent water supply systems have become more common in rural China and other developing countries. The accompanying hygiene risks require our more attention.

Methods: Based on the Grossman model, this paper conducted an IV-Oprobit model to investigate whether and how intermittent water supply affect rural residents' self-rated health status.

Protective effects of berberine against β-amyloid-induced neurotoxicity in HT22 cells via the Nrf2/HO-1 pathway.

Neuronal apoptosis has been found to have a pivotal role in the course of Alzheimer's disease (AD). Berberine (BBR), a potent antioxidant, occurs in plants such as Berberis, Phellodendron chinense, and Hydrastis canadensis. In this study, a neuronal apoptotic model was established in vitro using HT22 cells induced by Aβ to explore whether BBR contributes to protecting neurons against Aβ-induced neurotoxicity, as well as its potential mechanisms.

Revealed consumers' preferences for fresh produce attributes in Chinese online markets: A case of domestic and imported apples.

Background: The online market is getting popular today, and consumers' preferences about products are revealed differently in online and offline markets. Especially, fresh food purchasing online is very different from non-food products due to its unique features such as perishability, low cost and frequent purchases, low value-volume ratio, and highly relevance to safety and health. However, studies on online fresh food are rather few, and this study will fill the gap by investigating consumers' preferences for fresh food online purchasing.

Retraction notice to "The comparative study of clinical efficacy and safety of baclofen vs tolperisone in spasticity caused by spinal cord injury" [Saudi Pharm. J. 25 (2017) 655-659].

[This retracts the article DOI: 10.1016/j.jsps.

The comparative study of clinical efficacy and safety of baclofen vs tolperisone in spasticity caused by spinal cord injury.

In the present study we compared the clinical efficacy and safety of baclofen vs tolperisone in spasticity caused by spinal cord injury. A total of 150 patients were enrolled in the present study and were divided into two groups with 75 patients in each group, receiving baclofen or tolperisone, respectively. We used Modified Ashworth Scale, Medical research council scale, Barthel Index, and Coefficient of efficacy to measure clinical efficacy.

Evaluation of HE4 in the Diagnosis and Follow Up of Non-Small Cell Lung Cancers.

Background: The aim of our study was to evaluate the performance of HE4 in the diagnosis and follow up in patients with non-small cell lung cancer (NSCLC).

Methods: Serum levels of HE4, CEA, and Cyfra 21-1 were analyzed in 146 patients suspected with NSCLC and 30 healthy subjects to evaluate their diagnostic performance. A one year follow up was performed in 61 patients confirmed with NSCLC after surgery eradication at the interval of 1 month, 3 months, 6 months, and 12 months.

Tranexamic Acid Reduces Hidden Blood Loss in Patients Undergoing Total Knee Arthroplasty: A Comparative Study and Meta-Analysis.

Background: To explore the efficacy of tranexamic acid (TXA) on reducing hidden blood loss (HBL) in total knee arthroplasty (TKA) by conducting a comparative study and meta-analysis.

Material/methods: A total of 108 patients underwent TKA was equally distributed to experimental and control groups. The only difference between two groups was the administrations of 15 mg of TXA mixed in 100 mL normal saline for experimental group and 100 mL of normal saline for control group.

Effects of titanium dioxide nanoparticles coupled with diode laser on optical properties of in vitro normal and cancerous human lung tissues studied with optical coherence tomography and diffuse reflectance spectra.

The objective is to investigate the effects of two different sized (60 and 100 nm) titanium dioxide (TiO2) nanoparticles (NPs) penetration and accumulation in in vitro human normal lung (NL) tissue, lung squamous cell carcinoma (LSCC) tissue, and 650-nm diode laser-pretreated tissue on their optical properties studied with optical coherence tomography monitoring and diffuse reflectance (DR) spectra measurement. As with TiO2 NPs penetrating into the tissues, the intensities of DR of the samples increase, and then the enhancements of DR and the attenuation coefficients of the tissues were quantitatively calculated. The results suggest that 650-nm diode laser pretreatment increased the amounts of TiO2 NPs penetration and accumulation in NL and LSCC tissues, and the tissue optical properties were significantly influenced by accumulation of TiO2 NPs.

Influence of nanoparticles accumulation on optical properties of human normal and cancerous liver tissue in vitro estimated by OCT.

In this work, the potential use of nanoparticles as contrast agents by using spectral domain optical coherence tomography (SD-OCT) in liver tissue was demonstrated. Gold nanoparticles (average size of 25 and 70 nm), were studied in human normal and cancerous liver tissues in vitro, respectively. Each sample was monitored with SD-OCT functional imaging for 240 min.

In vitro assessment of effects of hyperglycemia on the optical properties of blood during coagulation using optical coherence tomography.

No published reports have demonstrated the capability of the optical coherence tomography technique for quantifying the optical coherence tomography signal slope, 1/e light penetration depth, and attenuation coefficient of hyperglycemic blood by an in vitro assessment. The purpose of this study was to investigate the effects of hyperglycemia on optical properties during in vitro blood coagulation by optical coherence tomography. Normal whole blood acted as the control group.

[Percutaneous screw fixation for the treatment of pelvic fractures under C-arm fluoroscopy].

Objective: To investigate the applications of percutaneous screw fixation for the treatment of pelvic fractures and its related surgical considerations.

Methods: From June 2010 to June 2012,19 patients with pelvic fractures were treated with percutaneous hollow screws. There were 13 males and 6 females, with an average age of 41 years (ranged from 22 to 58 years).

Continuous noninvasive monitoring of changes in human skin optical properties during oral intake of different sugars with optical coherence tomography.

The objective of this study was to evaluate the effects of blood glucose concentration (BGC) on in vivo human skin optical properties after oral intake of different sugars. In vivo optical properties of human skin were measured with a spectral domain optical coherence tomography (SD-OCT). Experimental results show that increase of BGC causes a decrease in the skin attenuation coefficient.

Noninvasive blood glucose monitoring during oral intake of different sugars with optical coherence tomography in human subjects.

The potential of OCT applied to noninvasive blood glucose monitoring has attracted significant efforts. In this work we investigated the feasibility of OCT in monitoring blood glucose during oral intake of different sugars in humans. Five groups of experiments were performed, in which different sugars were used.

Synergistic effect of hyperosmotic agents and sonophoresis on breast tissue optical properties and permeability studied with spectral domain optical coherence tomography.

Hyperosmotic agents have shown great potential in tissue optical clearing. However, the low efficiency of the permeation in biological tissues seriously restricts its application in reality. The synergy of sonophoresis as a penetration enhancer and hyperosmotic agents, 20% glucose (G) and 20% mannitol (M), in optical clearing has been investigated by analyzing the variation of the attenuation coefficients and the permeability coefficients.

Assessment of the effects of ultrasound-mediated glucose on permeability of normal, benign, and cancerous human lung tissues with the Fourier-domain optical coherence tomography.

The objective of this study was to evaluate the effects of ultrasound-mediated analyte diffusion on permeability of normal, benign, and cancerous human lung tissue in vitro and to find more effective sonophoretic (SP) delivery in combination with the optical clearing agents (OCAs) method to distinguish normal and diseased lung tissues. The permeability coefficients of SP in combination with OCAs diffusion in lung tissue were measured with Fourier-domain optical coherence tomography (FD-OCT). 30% glucose and SP with a frequency of 1 MHz and an intensity of 0.

Investigation of the permeability and optical clearing ability of different analytes in human normal and cancerous breast tissues by spectral domain OCT.

The potential of OCT applied for early breast cancer detection attracted significant efforts. The permeability coefficients and the percentages of OCT signal enhancement for normal and cancerous breast tissues have been investigated from the experiments of 20% glucose, 40% glucose, and 20% mannitol in vitro. Obtained results indicate that the permeability coefficient in breast cancer tissue is prominently larger than that in normal breast tissue, while the optical clearing effect is just the reverse to that for each agent.

Quantification of glucose diffusion in human lung tissues by using Fourier domain optical coherence tomography.

In this study, we report permeability coefficients of 30% glucose diffusion by the optical coherence tomography signal slope (OCTSS) method in four kinds of human lung tissue in vitro: normal lung tissue, benign granulomatosis lung tissue, squamous cell carcinoma and adenocarcinoma tumor. To quantify the permeability coefficient of the agent, the monitored region was 80 μm thickness at a tissue depth of ca 230 μm from the surface. The permeability coefficients of 30% glucose from 10 independent experiments were averaged and found to be (1.

[In vivo reflectance spectroscopy study of different clearing agents on human skin optical clearing].

The changes of skin tissue reflectance spectroscopy before and after being treated with the optical clearing agents of three different types of optical clearing within the wavelength rang of 400-1 000 nm, and the degree of changes in reflectance spectroscopy of each group skin during 0-60 min at 580 nm in vivo were real-time dynamically researched. The reflectance spectroscopy of skin tissue before and after being dealt by the optical clearing agents of glycerol, glucose and propylene glycol was measured using a USB-4000 fiber spectrophotometer at 0, 10, 20, 30, 40, 50 and 60 min. The results showed that the reflectance spectral intensity was distinctly decreased, but the reflectance was significantly increased gradually with the time prolonged.

In vivo comparison of the optical clearing efficacy of optical clearing agents in human skin by quantifying permeability using optical coherence tomography.

The objective of this work is to quantify and compare the optical clearing efficacy of glucose, propylene glycol, glycerol solutions through the human skin tissue in vivo by calculating permeability coefficient of three solutions. Currently, the permeability coefficient of agent in tissues was extracted from optical coherence tomography (OCT) amplitude data mainly through the OCT signal slope and the OCT amplitude methods. In this study, we report the OCT attenuation coefficient method which is a relatively novel and rarely reported methodology to measure the permeability coefficient during the optical skin clearing procedure.

[Serum proteomics profiling for diagnosis of myasthenia gravis].

Objective: To identify the significant protein peaks and establish the diagnostic model of myasthenia gravis (MG) by serum proteomics profiling analysis.

Methods: The serum samples from 56 MG patients and 16 healthy controls were detected by the technology of surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). The differentially expressed protein peaks were identified to establish a MG diagnostic model.

[Gastric cancer detection using kubelka-Munk spectral function of DNA and protein absorption bands].

Differential diagnosis for epithelial tissues of normal human gastric, undifferentiation gastric adenocarcinoma, gastric squamous cell carcinomas, and poorly differentiated gastric adenocarcinoma were studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 250 to 650 nm, pathological changes of gastric epithelial tissues induced that there were significant differences in the averaged value of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log[f(r infinity)] of the DNA absorption bands at 260 nm between epithelial tissues of normal human stomach and human undifferentiation gastric cancer, between epithelial tissues of normal human stomach and human gastric squamous cell carcinomas, and between epithelial tissues of normal human stomach and human poorly differentiated cancer.

[Colon adenoma detection using Kubelka-Munk spectral function of DNA and protein bands].

Differential diagnosis of human colon adenoma was studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 590 to 1 064 nm pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log [f(r infinity)] of the DNA absorption bands at 260 nm between normal and adenomatous colon epithelial tissues, and the differences were 218% (p < 0.

Serum protein profiles in myasthenia gravis.

Background: The diagnosis of myasthenia gravis (MG) remains challenging. We performed a proteome-wide search for potential serum protein diagnostic markers for MG using surface-enhanced laser desorption/ionization (SELDI) time-of-flight mass spectrometry (TOFMS).

Methods: Proteomic spectra from 80 MG patients and 80 healthy individuals were generated by SELDI.